AAT Bioquest社 Improving Accuracy In Measuring NADP/NADPH

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Improving Accuracy In Measuring NADP/NADPH
NADP/NADPH is the least abundant of the nicotinamide adenine dinucleotide pairs. NADPH is associated with biosynthesis of macromolecules, providing the reducing power necessary for those synthetic reactions. Increased biosynthesis is characteristic of rapidly proliferating cells, such as cancer cells, and NADPH is considered a key molecule produced as a result of cancer metabolism. as well as Reactive Oxygen Species (ROS). Many traditional NADP/NADPH assays are done by monitoring absorption at 340 nm, which requires expensive quartz microplates. Unfortunately, this method suffers low sensitivity and high interference. • Use an assay that uses a longer wavelength for better signal/noise ratio. • To improve assay sensitivity, fluorimetric format allows lower interference. Contact us for any help in choosing the optimal kit or pre-labeled antibody from our catalog.	Total NADPH and NADP dose response: Amplite® Colorimetric NADP/NADPH Ratio Assay Kit was used to measure total NADP/NADPH amount. Equal amounts of NADP and NADPH mixture were treated with or without NADP extraction solution for 15 minutes, and then neutralized with extraction solution at room temperature. The signal was read at 460 nm. NADP/NADPH Digital Catalog
Resources & Information AAT Bioquest has a wide range of resources and specialized products for researchers, from Application Notes & FAQs to spectral profiles. Related Digital Catalogs: • Amplite® Reagents and Assay Kits • Cell Metabolism • Reactive Oxygen Species (ROS) AssayWise Newsletter Research Articles: • Rapid & Sensitive Detection of NAD, NADH, NADP & NADPH Using Amplite Assay Kits • Total Solution for NAD/NADH & NADP/NADPH Detection Customer Favorites Amplite® Assay Kits: • Colorimetric Total NADP and NADPH Assay • Fluorimetric NADP/NADPH Ratio Assay	Featured Citation NADP+/NADPH Ratio: The ratio of NADP+/NADPH under normoxia and hypoxia in shScr or shACC1 H1975 cells was calculated with the Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit. Source: ACLY and ACC1 Regulate Hypoxia-Induced Apoptosis by Modulating ETV4 via α-ketoglutarate Keenan, et al., PLOS (2015).

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