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AAT Bioquest社 Using Phalloidin's Smaller Size for Improved Actin Labeling Density and Imaging





Labeling Actin: Phalloidin vs. Antibody Conjugates

Fluorescent-labeled phalloidin is enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. In comparison to antibodies, phalloidin-dye conjugates are smaller, so labeling does not impede cell function. This small size also permits much denser labeling, which allows for more detailed images at higher resolutions.
Although a wide range of options are available, for multicolor imaging applications green color conjugates are the most popular. Fluorescein is a classic choice, with Alexa Fluor® 488 and the newest high performing Phalloidin-iFluor® 488 conjugates providing brighter signal with greater photostability.
For help in transitioning your workflow from older protocols for modern labels, our scientists are available for technical support.


Visit The Phalloidin Conjugates Catalog

Using_Phalloidin's_Smaller_Size_for_Improved_Actin_Labeling_Density_and_Imaging_2.pngCell Types & Phalloidin Staining:
A) CPA cell labeled with Phalloidin iFluor® 488, and co-stained with lysosomal dye LysoBrite™ Red and nuclei stain Nuclear Blue™ DCS1. B) HeLa cells labeled with Phalloidin-iFluor® 350 and co-stained with nuclei stain Nuclear Red™ DCS1. C) HeLa cells labeled with Phalloidin-iFluor® 555, and co-stained with mitochondria stain MitoLite™ Green FM, nuclei stain Nuclear Blue™ DCS1, and plasma membrane stain Cellpaint™ Deep Red.

Resources & Information


AAT Bioquest has a wide range of resources and specialized products for researchers, from Application Notes to FAQs.
Assaywise Letters Research Article:
Fluorescent Phalloidin: A Practical Stain for Visualizing Actin Filaments
Online Tools:
Interactive Spectrum Viewer
Related Digital Catalogs:
Antibody and Protein Labeling
iFluor® Dyes and Kits

Using_Phalloidin's_Smaller_Size_for_Improved_Actin_Labeling_Density_and_Imaging_3.jpgMulticolor Fluorescent Imaging: HeLa cells were fixed in 4% formaldehyde, co-labeled with mitochondria dye MitoLite™ Red FX600 (red), actin filaments with Phalloidin- iFluor® 488 Conjugate (green), and nuclei were stained with Nuclear Blue™ DCS1 (blue).

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