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AAT Bioquest has developed the largest collection of calcium indicators, such as our high-demand Fluo-8®, Cal-520®, and Calbryte™ probes, as well as calcium flux assays to track calcium mobilization with intense fluorescent signals and in a variety of wavelength options ranging from ultraviolet to infrared.

Our classic green as well as long-wavelength probes, including our unique NIR calcium indicator Cal-770™, are manufactured in-house for dependable supply.

Choosing the right probe depends mainly on the experiment. For assistance with product selection, recommended protocols, or other experimental concerns, contact the distributor.

Ratiometric Calcium Indicators:   Dye Filter Set Kd Fura-2, AM Fura 2 145 nM Fura-FF, AM Fura 2 5.5 µM Fura-8™, AM Fura 2 260 nM Fura-8FF™, AM Fura 2 6 µM Fura-10™, AM Fura 2 260 nM Fura Red, AM Fura Red 400 nM Cal Red™ R525/650 AM Fura Red 330 nM

Performance Comparison: ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells measured with Fura-2 AM, Fura-8™ AM and Fura-10™ AM.

Upgraded Single-Wavelength Green Calcium Indicators:

These indicators exhibit significant calcium-dependent changes in fluorescence intensity at saturating calcium levels without shifting either their absorbance or emission wavelengths. 

• Cal-520® AM has both high sensitivity and >100 times fluorescence enhancement when bound to calcium.
• Improved signal/background ratio and intracellular retention properties make Calbryte™ 520 AM the most robust indicator for evaluating GPCR and calcium channel targets
• Fluo-8® is a novel, high-performing green calcium indicator which incorporates the same fluorescein core as Fluo-3 and Fluo-4.

The majority of our indicators are available as membrane-permeant AM esters, and membrane-impermeant salt derivatives and dextran conjugates. We also offer custom conjugations as well as stand-alone reagents for researchers with highly unique requirements.