※ Three mutant types (V367F, W436R, and D364Y) in the RBD showed higher binding affinity to human ACE2.

Article: Emergence of RBD mutations in circulating SARS-CoV-2 strains enhancing the structural 1 stability and human ACE2 receptor affinity of the spike protein
Click for the full article >>

※ ACROBiosystems has validated the binding affinity between the mutant SARS-CoV-2 RBDs (V367F, W436R, and N354D, D364Y) and the human ACE2. Protocols offered for free.

ELISA

The enhancement of the binding affinity of the mutant SARS-CoV-2 RBDs was validated by ELISA assay.

The EC50 of RBD (V367F), RBD (W436R), RBD (N354D, D364Y) and RBD binding to human ACE2 was 0.31nM, 0.89nM, 0.97nM and 1.47nM, respectively.

SPR

The enhancement of the binding affinity of the mutant SARS-CoV-2 RBDs was validated by SPR.

The KD of RBD (V367F), RBD (W436R), RBD (N354D, D364Y) and RBD binding to human ACE2 was 4.33nM, 6.96nM, 7.57nM and 13.1nM.

BLI

The enhancement of the binding affinity of the mutant SARS-CoV-2 RBDs was validated by BLI.

The KD of RBD (V367F), RBD (W436R), RBD (N354D, D364Y) and RBD binding to human ACE2 was 5.5nM, 6.85nM, 6.33nM and 24.4nM, respectively.

RBD Product List