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ACRObiosystems社 Mutations in S RBD affecting the binding activity to ACE2

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Mutations_in_S_RBD_affecting_the_binding_activity_to_ACE2_2.jpg

Three mutant types (V367F, W436R, and D364Y) in the RBD showed higher binding affinity to human ACE2.

Article: Emergence of RBD mutations in circulating SARS-CoV-2 strains enhancing the structural 1 stability and human ACE2 receptor affinity of the spike protein
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 ACROBiosystems has validated the binding affinity between the mutant SARS-CoV-2 RBDs (V367F, W436R, and N354D, D364Y) and the human ACE2. Protocols offered for free.

ELISA

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The enhancement of the binding affinity of the mutant SARS-CoV-2 RBDs was validated by ELISA assay.

The EC50 of RBD (V367F), RBD (W436R), RBD (N354D, D364Y) and RBD binding to human ACE2 was 0.31nM, 0.89nM, 0.97nM and 1.47nM, respectively.

SPR

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The enhancement of the binding affinity of the mutant SARS-CoV-2 RBDs was validated by SPR.

The KD of RBD (V367F), RBD (W436R), RBD (N354D, D364Y) and RBD binding to human ACE2 was 4.33nM, 6.96nM, 7.57nM and 13.1nM.

BLI

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The enhancement of the binding affinity of the mutant SARS-CoV-2 RBDs was validated by BLI.

The KD of RBD (V367F), RBD (W436R), RBD (N354D, D364Y) and RBD binding to human ACE2 was 5.5nM, 6.85nM, 6.33nM and 24.4nM, respectively.

RBD Product List

Molecule

Cat. No.

Species

Product Description

S protein RBD

SPD-C82E9

SARS-CoV-2

Biotinylated SARS-CoV-2 (COVID-19) S protein RBD,   His,Avitag™ (MALS   verified)

S protein RBD

SPD-C5255

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD, Fc Tag (MALS   verified)

S protein RBD

SPD-C52H3

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS   verified)

S protein RBD

SPD-C5259

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc   Tag

S protein RBD

SPD-S52H4

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

S protein RBD

SPD-S52H5

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag

S protein RBD

SPD-S52H3

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (N354D, D364Y),   His Tag

S protein RBD

SPD-S52H7

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag

S protein RBD

SPD-S52H8

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag

S protein RBD

SPD-S52H6

SARS

SARS S protein RBD, His Tag (MALS verified)

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