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ACRObiosystems社 Which animal model can help defeat COVID-19?

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The spike protein of SARS-CoV-2 facilitates viral entry into the target cell exploiting the Angiotensin-converting enzyme 2 (ACE2) receptor. The ACE2 gene is highly conserved in mammalian animals at the DNA and peptide level, which makes them the potential hosts for SARS-CoV-2.

A recent study found that the ACE2 gene was discovered in 12 common mammals. Among these 12 mammals, humans are the main host of SARS-CoV-2; mice are commonly used experimental animal models; bats and pangolins are suspected to be the intermediate host; cats, dogs, ferrets, and hamsters are pets; cattle, pigs, rabbits, and goats are common livestock. [1] Besides these 12 mammals, paguma larvata, an important host of SARS, also has ACE2 expression [2].

At ACROBiosystems, we verified the binding activity between different species of ACE2 and S1 or S RBD proteins using ELISA/SPR/BLI. Check out which one will be the suitable animal model.

  Bioactivity-BLI

We investigated the binding activity between the ACE2 of human, cynomolgus or paguma larvata, and the S RBD of SARS-CoV-2 using BLI. The binding activities of human and cynomolgus ACE2 to the S RBD of SARS-CoV2 are much higher than paguma larvata ACE2.

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  Bioactivity-ELISA

Paguma larvata is an important host of SARS. Its ACE2 receptor protein also showed a very good binding ability to the S protein of SARS-CoV-2, though not comparable to human and cynomolgus ACE2. 

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  Bioactivity-SPR

An SPR method was also used to investigate the binding affinity between paguma larvata ACE2 and S RBD or S1 protein of SARS and SARS-CoV-2. Based on the results, we concluded that paguma larvata ACE2 is more susceptible to SARS.

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Product List 

Molecule

Cat. No.

Species

Product description

ACE2

AC2-H82E6

Human

Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)

AC2-H82F9

Human

Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™

AC2-H5257

Human

Human ACE2 / ACEH Protein, Fc Tag (MALS verified)

AC2-H52H8

Human

Human ACE2 / ACEH Protein, His Tag (MALS verified)

AC2-C52H7

Cynomolgus

Cynomolgus ACE2 / ACEH Protein, His Tag

AC2-P5248

Paguma larvata

Paguma larvata ACE2 / ACEH Protein, His Tag

AC2-M5248

Mouse

Mouse ACE2 / ACEH Protein, His Tag (MALS verified)

AC2-R5246

Rat

Rat ACE2 / ACEH Protein, His Tag (MALS verified)

AC2-C5245

Canis

Canis ACE2 / ACEH Protein, His Tag

S protein

SPN-C52H4

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag

SPN-C52H8

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag, active trimer (MALS verified)

SPN-S52H5

SARS

SARS S protein (R667A), His Tag

S1 protein

S1N-C52H3

SARS-CoV-2

SARS-CoV-2 (COVID-19) S1 protein, His Tag

S1N-C82E8

SARS-CoV-2

Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His, Avitag™ (MALS verified)

S1N-S52H5

SARS

SARS S1 protein, His Tag (MALS verified)

S1N-C52H4

SARS-CoV-2

SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)

S1N-C5255

SARS-CoV-2

SARS-CoV-2 (COVID-19) S1 protein, Fc Tag

S1N-C5257

SARS-CoV-2

SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag

S protein RBD

SPD-C82E9

SARS-CoV-2

Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)

SPD-C5255

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD, Fc Tag (MALS verified)

SPD-S52H6

SARS

SARS S protein RBD, His Tag (MALS verified)

SPD-C52H3

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)

SPD-C5259

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag

SPD-S52H4

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

SPD-S52H5

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag

SPD-S52H7

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag

SPD-S52H8

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag

SPD-S52H3

SARS-CoV-2

SARS-CoV-2 (COVID-19) S protein RBD (N354D, D364Y), His Tag

Reference
[1] Sun et al., 2020 Atlas of ACE2 gene expression in mammals reveals novel insights in transmisson of SARS-Cov-2, doi: http://life.acrobiosystems.cn/e/535472/10-1101-2020-03-30-015644/2dvml2l/919136533?h=gTzj8imS4yO1h7_OQo8L-d7H5Io6YwQ2DgGxOe_YAUQ

[2] Stawiski et al., 2020 Human ACE2 receptor polymorphisms predict SARS-CoV-2 susceptibility. doi: http://life.acrobiosystems.cn/e/535472/10-1101-2020-04-07-024752/2dvml2n/919136533?h=gTzj8imS4yO1h7_OQo8L-d7H5Io6YwQ2DgGxOe_YAUQ

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