Allele Biotechnology社黄緑色蛍光タンパク質 mNeonGreen

mNeonGreenは、頭索類Branchiostoma lanceolatum由来の四量体蛍光タンパク質から得られた単量体の黄緑色蛍光タンパク質で、単量体の緑色蛍光タンパク質および黄色蛍光タンパク質の中で最高の輝度を有します。従来のイメージングと同様に、超解像分子イメージングにも優れたタグとして利用できます。また、蛍光共鳴エネルギー移動（FRET）におけるシアン色蛍光タンパク質のアクセプターとして有効です。

詳細情報　＞＞

mNeonGreen情報　https://reagents.allelebiotech.com/mneongreen-1/
ライセンスについて　https://reagents.allelebiotech.com/sublicensing/
ライセンス取得施設　https://reagents.allelebiotech.com/fluorescent-protein-active-licenses/

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メーカー情報

For a span of 20 years, researchers have by and large looked to Aequorea victoria Green Fluorescent protein (GFP) for their imaging, and tracking needs. Over this time period, the development of GFP has elicited a wide spectrum of colors and optimized versions for a multitude of applications. As imaging moves into the super resolution era, most common fluorescent proteins, including GFP, may not possess certain characteristics beneficial for super resolution applications. With this in mind, Allele now offers a new high performance monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein to date, and is an excellent fusion tag for traditional imaging as well as stochastic single-molecule super resolution imaging. Lastly it is a stellar fluorescence resonance energy transfer (FRET) acceptor for cyan fluorescent proteins. For more information on mNeonGreen's properties view our publication, "A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum" in the journal Nature Methods.

■ mNeonGreen absorbance, excitation, and emission

■ mNeonGreen Basic Properties

Excitation Max (nm)	506
Emission Max (nm)	517
Quantum Yield	0.8
Extinction coefficient	116,000
Brightness	92.8
pKa	5.7

■ Super Resolution Imaging Localization Data

Fluorescent Protein	# Molecules Localized	# Photons per Molecule	PSF Width (nm)
mNeonGreen	33,783	663 ± 259	167.3 ± 34.1
Clover	15,281	408 ± 263	176.4 ± 57.6
mEGFP	6,803	523 ± 261	165.8 ± 37.1

■ Fluorescence imaging of mNeonGreen fusion vectors

Actin	Plasma Membrane	Microtubules	Replication