Alomone Labs社では、イオンチャネル（Ion channel）に対する抗体のセットを販売しています。セット内容は、TRPC Channel Antibody Explorer Kitを例に挙げると、TRPC Channel関連の12種類の抗体がセットされています。コントロールとしてそれぞれの抗体のペプチド抗原が添付されます。 

 

• AMPA Receptor Antibody Explorer Kit （Cat# AK-213）
• BK_ca Channel Antibody Explorer Kit （Cat# AK-217）
• SK (Non-BK_ca) Channel Antibody Explorer Kit （Cat# AK-218）
• CNGA Antibody Explorer Kit （Cat# AK-204）
• HCN Antibody Explorer Kit （Cat# AK-205）
• K_ir Channel Antibody Explorer Kit （Cat# AK-200）
• hERG and hERG-Related Channel Antibody Explorer Kit （Cat# AK-222）
• KCNQ Channel Antibody Explorer Kit （Cat# AK-221）
• Shaker (K_V1) Channel Antibody Explorer Kit （Cat# AK-219）
• Shaker (K_V)-Related Channel Antibody Explorer Kit （Cat# AK-220）
• Two-Pore Domain K⁺ Channel Antibody Explorer Kit （Cat# AK-202）
• P2X Receptor Antibody Explorer Kit （Cat# AK-201）
• Store-Operated Ca²⁺ Channel Antibody Explorer Kit （Cat# AK-209）
• TRPC Channel Antibody Explorer Kit （Cat# AK-212）
• TRPM Channel Antibody Explorer Kit （Cat# AK-210）
• TRPV Channel Antibody Explorer Kit （Cat# AK-211）
• TTX-Resistant Na_V Channel Antibody Explorer Kit （Cat# AK-223）
• TTX-Sensitive Na_V Channel Antibody Explorer Kit （Cat# AK-224）
• NMDA Receptor Antibody Explorer Kit  （Cat# AK-214）
• L-Type Ca_V Channel Antibody Explorer Kit （Cat# AK-215）
• Non-L-Type Ca_V Channel Antibody Explorer Kit （Cat# AK-216）

 

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メーカー情報 

  

 ● AMPA Receptor Antibody Explorer Kit   

 

The ionotropic glutamate receptors are divided into three main categories: AMPA, NMDA (N-methyl-D-aspartate) and Kainate Receptors.

AMPA Receptors consist of four closely related genes with a high sequence homology. Their related products, GluR1-GluR4 subunits combine in tetramers in different stoichiometries¹ which determine their channel function. These receptors, like many other cellular proteins undergo post-translational modifications. Such modifications influence protein-protein interactions, trafficking, internalization and their accumulation in the Golgi. Also, post-translational modifications are also involved in regulating LTP-driven incorporation of AMPA Receptors into the post-synaptic membrane (extensively reviewed in reference 2).

AMPA Receptors are responsible for the primary depolarization in glutamate-mediated neurotransmission. in situ hybridization and immunocytochemistry studies show that AMPA Receptors are mainly distributed in the brain. These receptors play important roles in synaptic plasticity, excitatory neurotransmission, LTP and LTD effects, and take part in learning and memory formation^3-6. The important functions AMPA Receptors have acquired, also imply that deregulation of these receptors leads to pathologies.

Alomone Labs is pleased to offer the AMPA Receptor Antibody Explorer Kit (#AK-213). The Explorer Kit contains AMPA receptor antibodies with their respective peptide control antigen. An ideal tool for screening purposes.

Compound: 

Anti-AMPA Receptor 1 (GluA1) (extracellular)     #AGC-004     1 x 50 µl
Anti-AMPA Receptor 1 (GluA1) (extracellular)     #AGP-009     1 x 50 µl
Anti-AMPA Receptor 2 (GluA2) (extracellular)     #AGC-005     1 x 50 µl

 

 

 ● BK_ca Channel Antibody Explorer Kit   

 

The K_Ca1.1 channel (also known as BK_Ca, Maxi K⁺ or slo) is part of a structurally diverse group of K⁺ channels that are activated by an increase in intracellular Ca²⁺. K_Ca1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca²⁺ and by membrane depolarization.  In addition, the K_Ca1.1 channel structurally differs from the other Ca²⁺-dependent K⁺ channels.

While the latter group has a topology that resembles that of the voltage-dependent K⁺ channels, the K_Ca1.1 channel has an extracellular N-terminus domain as well as an additional transmembrane domain.

K_Ca1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect between intracellular Ca²⁺ signaling pathways and membrane excitability.
 
Indeed, K_Ca1.1 channels play a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.

Alomone Labs is pleased to offer the BK_Ca Channel Antibody Explorer Kit (#AK-217). This Explorer Kit includes antibodies targeted against BK_Ca channels and auxiliary subunits along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-K_Ca1.1 (1097-1196)     #APC-021     1 x 50 µl
Anti-K_Ca1.1 (1184-1200)     #APC-107     1 x 50 µl
Anti-K_Ca1.1 (BK_Ca) (extracellular)     #APC-151     1 x 50 µl
Anti-sloβ1 (KCNMB1)     #APC-036     1 x 50 µl
Anti-sloβ2 (KCNMB2)     #APC-034     1 x 50 µl
Anti-sloβ3 (KCNMB3) (extracellular)     #APC-068     1 x 50 µl
Anti-sloβ4 (KCNMB4)     #APC-061     1 x 50 µl
Anti-KCNE4 (MiRP3)     #APC-129     1 x 50 µl

 

 

 ● SK (Non-BK_ca) Channel Antibody Explorer Kit   

 

Ca²⁺ dependent K⁺ (K_Ca) channels are divided according to biophysical properties and gene homology into two main groups. K_Ca were first divided according to their single channel conductance, which represents the speed by which the K⁺ passes via the open channel. The first group consists of small and intermediate conductance channels (SK and IK respectively, the KCNN gene family). The second group is comprised of large/big conductance channels (called BKCa or maxiK, encoded by the slo or KCNMA1 gene)¹.

Alomone Labs is pleased to offer the SK (Non-BK_Ca) Channel Antibody Explorer Kit (#AK-218). The Explorer Kit contains Non-K_Ca channel antibodies, ideal for screening purposes.

Compound:

Anti-K_Ca2.1 (SK1, SK_Ca1)     #APC-039     1 x 50 µl 
Anti-K_Ca2.2 (SK2)     #APC-028     1 x 50 µl 
Anti-K_Ca2.3 (SK3) (N-term)     #APC-025     1 x 50 µl
Anti-K_Ca2.3 (C-term)     #APC-103     1 x 50 µl
Anti-K_Ca3.1 (SK4, IK_Ca1)     #APC-064     1 x 50 µl
Anti-K_Ca3.1 (SK4) (extracellular)     #ALM-051     1 x 50 µl
Anti-K_Ca4.1 (Slack)     #APC-124     1 x 50 µl
Anti-K_Ca4.2 (Slick)     #APC-126     1 x 50 µl

 

  

 ● CNGA Antibody Explorer Kit   

 

Cyclic Nucleotide-Gated (CNG) channels belong to the superfamily of voltage-gated ion channels. Although permeable to various ions such as the monovalent Na⁺ and K⁺ ions, and the divalent Ca²⁺ ion, they are gated by the intracellular binding of the cyclic nucleotides cAMP and cGMP and not by voltage per se (CNGs bind preferably to cGMP)¹.

Six channels form this subfamily: The A subunit (CNGA1-4) and the B subunit (CNGB1 and CNGB3). Functional entities are formed by the assembly of four subunits. Each subunit consists of six membrane spanning domains, where the fourth transmembrane domain is highly positively charged, a pore domain between transmembrane domains five and six, and intracellular N- and C-termini. The cyclic nucleotide binding domain is located in the C-terminal region and is responsible for the channel gating upon cyclic nucleotide binding¹.

These channels are highly expressed in retinal photoreceptors and olfactory neurons where their role has been extensively studied². CNG channels have also been detected in brain, testis and kidney although their role in these tissues has yet to be unraveled².

Alomone Labs is pleased to offer the CNGA Antibody Explorer Kit (#AK-204). This Explorer Kit includes antibodies targeted against CNGAs along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-CNGA1     #APC-071     1 x 50 µl

 

 

 ● HCN Antibody Explorer Kit   

 

Hyperpolarization-activated cation currents (I_h) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity^1,2.

The I_h currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.

Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus². The HCN subunits can form functional homomers and can also co-assemble into functionally heteromers².

The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The channels HCN1-4 mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.

Alomone Labs is pleased to offer the HCN Antibody Explorer Kit (#AK-205). The Explorer Kit contains HCN antibodies with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-HCN1     #APC-056     1 x 50 µl

 

  

 ● K_ir Channel Antibody Explorer Kit   

 

K_ir1.1 (ROMK1) was the first member of the family of inward rectifying K⁺ channels to be cloned¹. The family includes 15 members that are structurally and functionally different from the voltage-dependent K⁺ channels.

The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K⁺ channels the functional unit for the K_ir channels is composed of four subunits that can assembly as either homo or heterotetramers.

K_ir channels are characterized by a K⁺ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K⁺ homeostasis².

Alomone Labs is pleased to offer the K_ir Channel Antibody Explorer Kit (#AK-200). This Explorer Kit includes antibodies targeted against K_ir channels along with their respective peptide control antigen (where applicable). An ideal tool for screening purposes.

Compound:

Anti-K_ir1.1 (ROMK1)     #APC-001     1 x 50 µl

 

  

 ● hERG and hERG-Related Channel Antibody Explorer Kit   

 

The EAG family of voltage-gated K⁺ channels can be subdivided into three distinct groups based on sequence homology. They are the eag (K_v10) with two members, the eag-related channels (erg or K_v11) and the eag-like K⁺ channels (elk or K_v12) with three members each.

Alomone Labs is pleased to offer the hERG and hERG-Related Channel Antibody Explorer Kit (#AK-222). This Explorer Kit includes antibodies targeted against K_V10, K_V11 and K_V12 channels along with their respective peptide control antigen. An ideal tool for screening purposes.


Compound:

Anti-K_V10.1 (EAG-1)     #APC-104     1 x 50 µl
Anti-K_V10.2 (EAG-2)     #APC-053     1 x 50 µl
Anti-hK_v11.1 (HERG)     #APC-062     1 x 50 µl
Anti-K_V11.1 (erg1)     #APC-016     1 x 50 µl
Anti-K_V11.1 (HERG) (extracellular)     #APC-109     1 x 50 µl
Anti-K_V11.2 (erg2)     #APC-114     1 x 50 µl
Anti-K_V11.3 (erg3)     #APC-112     1 x 50 µl
Anti-K_V12.1 (Elk1)     #APC-113     1 x 50 µl
Anti-K_V12.3 (Elk3)     #APC-116     1 x 50 µl 

 

 

 ● KCNQ Channel Antibody Explorer Kit   

 

The KCNQ family of voltage-gated K⁺ channels includes 5 known members: KCNQ1 to KCNQ5.  Structurally, the KCNQ family belongs to the six transmembrane domain category of K⁺ channels.  KCNQ family members can form either homomultimeric or heteromultimeric channels with different functional consequences. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K⁺ neuronal current that is strongly inhibited by the activation of the M₁ subtype of the muscarinic acetylcholine receptor.
K_V7.5 is also expressed in visceral smooth muscle⁴ and skeletal muscle.

Alomone Labs is pleased to offer the KCNQ Channel Antibody Explorer Kit (#AK-221). This Explorer Kit includes antibodies targeted against K_V7 channels and subunits along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-K_V7.1 (KCNQ1)     #APC-022     1 x 50 µl
Anti-K_V7.2 (KCNQ2)     #APC-050     1 x 50 µl
Anti-K_V7.3 (KCNQ3)     #APC-051     1 x 50 µl
Anti-K_V7.5     #APC-155     1 x 50 µl
Anti-KCNE1 (IsK)     #APC-008     1 x 50 µl
Anti-KCNE2 (MiRP1)     #APC-054     1 x 50 µl
Anti-KCNE3 (MiRP2)     #APC-118     1 x 50 µl
Anti-KCNE4 (MiRP3)     #APC-129     1 x 50 µl

 

 

 ● Shaker (K_V1) Channel Antibody Explorer Kit   

 

K⁺ selective channels are some of the most widespread ion trafficking molecules in living organisms, with more than 70 genes encoding different K⁺ channels in humans. K_V channels fall into one of the two classical categories of delayed rectifier (DR) and A-type. Delayed rectifier was the original name attributed to voltage-dependent K⁺ channels due to their delayed activation in squid giant axons. A-type channels are low voltage-activated, fast inactivating (therefore, transient) K⁺ channels. Specific K_V toxins are often used to dissect the particular contribution of different subunits to native currents.

Alomone Labs is pleased to offer the Shaker (K_V1) Channel Antibody Explorer Kit (#AK-219). This Explorer Kit includes antibodies targeted against K_V1 channels along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-K_V1.1     #APC-009     1 x 50 µl
Anti-K_V1.2     #APC-010     1 x 50 µl
Anti-K_V1.3     #APC-002     1 x 50 µl
Anti-K_V1.3 (extracellular)     #APC-101     1 x 50 µl
Anti-K_V1.3 (extracellular)     #AGP-005     1 x 50 µl
Anti-K_V1.4     #APC-007     1 x 50 µl
Anti-K_V1.5     #APC-004     1 x 50 µl
Anti-K_V1.5 (extracellular)     #APC-150     1 x 50 µl
Anti-K_V1.6     #APC-003     1 x 50 µl
Anti-K_V1.7     #APC-063     1 x 50 µl
Anti-K_V1.8     #APC-157     1 x 50 µl
Anti-K_Vβ1     #APC-127     1 x 50 µl
Anti-K_Vβ2     #APC-117     1 x 50 µl

 

 

 ● Shaker (K_V)-Related Channel Antibody Explorer Kit   

 

K⁺ selective channels are some of the most widespread ion trafficking molecules in living organisms, with more than 70 genes encoding different K⁺ channels in humans. K_V channels fall into one of the two classical categories of delayed rectifier (DR) and A-type. Delayed rectifier was the original name attributed to voltage-dependent K⁺ channels due to their delayed activation in squid giant axons. A-type channels are low voltage-activated, fast inactivating (therefore, transient) K⁺ channels. Specific K_V toxins are often used to dissect the particular contribution of different subunits to native currents.

Alomone Labs is pleased to offer the Shaker (K_V)-Related Channel Antibody Explorer Kit (#AK-220). This Explorer Kit includes antibodies targeted against K_V2, K_V3 and K_V4 channels along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-K_V2.1     #APC-012     1 x 50 µl
Anti-K_V2.2     #APC-120     1 x 50 µl
Anti-K_V3.1b     #APC-014     1 x 50 µl
Anti-K_V3.2     #APC-011     1 x 50 µl
Anti-K_V3.3     #APC-102     1 x 50 µl
Anti-K_V3.4     #APC-019     1 x 50 µl
Anti-K_V4.1     #APC-119     1 x 50 µl
Anti-K_V4.2     #APC-023     1 x 50 µl
Anti-K_V4.3     #APC-017     1 x 50 µl
Anti-KChIP1     #APC-141     1 x 50 µl
Anti-KChIP3     #APC-143     1 x 50 µl
Anti-KCNE4 (MiRP3)     #APC-129     1 x 50 µl

 

 

 ● Two-Pore Domain K⁺ Channel Antibody Explorer Kit   

 

Two pore domain K⁺ channel (K_2P) family consists of 15 members which are further divided to six subfamilies on the basis of structural and functional properties. All family members are tightly controlled by various chemical and physical stimuli such as pH, oxygen tension, lipids, mechanical stretch, G-protein coupled receptors, transmitters and other endogenous modulators, and are targets for clinically important compounds such as volatile anesthetic. These channels are also known as "leak channels". This name is given since they are open across the physiological voltage range, show little voltage or time dependence and are believed to underlie many of the K⁺ leak currents. This leak is active during the rest period of excitable cells, stabilizes membrane potential below firing threshold and hasten repolarization in many neurons including cerebellar granular neurons.

Alomone Labs is pleased to offer the Two-Pore Domain K⁺ Channel Antibody Explorer Kit (#AK-202). This Explorer Kit includes antibodies targeted against K_2P channels along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-K_2P1.1 (TWIK-1) (extracellular)     #APC-110     1 x 50 µl
Anti-K_2P2.1 (TREK-1)     #APC-047     1 x 50 µl
Anti-K_2P3.1 (TASK-1)     #APC-024     1 x 50 µl
Anti-K_2P4.1 (TRAAK)     #APC-108     1 x 50 µl
Anti-K_2P5.1 (TASK-2)     #APC-037     1 x 50 µl
Anti-K_2P6.1 (TWIK-2)     #APC-040     1 x 50 µl
Anti-K_2P9.1 (TASK-3)     #APC-044     1 x 50 µl
Anti-K_2P10.1 (TREK-2)     #APC-055     1 x 50 µl
Anti-K_2P13.1 (THIK-1) (extracellular)     #APC-121     1 x 50 µl
Anti-K_2P18.1 (TRESK) (extracellular)     #APC-122     1 x 50 µl 

 

 

 ● P2X Antibody Explorer Kit   

 

The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.

The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.

Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned^1-3. All P2X receptor subtypes share the same structure of intracellular N and C-termini two membrane-spanning domains and a large extracellular loop.

All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex^4-9.

The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system⁴. The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis^3,4,10,11.

Alomone Labs is pleased to offer the P2X Antibody Explorer Kit (#AK-201). This Explorer Kit includes antibodies targeted against P2X receptors along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-P2X1 Receptor     #APR-001     1 x 50 µl     
Anti-P2X1 Receptor (extracellular)     #APR-022     1 x 50 µl
Anti-P2X2 Receptor     #APR-003     1 x 50 µl
Anti-P2X2 Receptor (extracellular)     #APR-025     1 x 50 µl
Anti-P2X3 Receptor     #APR-016     1 x 50 µl
Anti-P2X3 Receptor (extracellular)     #APR-026     1 x 50 µl
Anti-P2X4 Receptor     #APR-002     1 x 50 µl
Anti-P2X4 Receptor (extracellular)     #APR-024     1 x 50 µl
Anti-P2X5 Receptor     #APR-005     1 x 50 µl
Anti-P2X6 Receptor     #APR-013     1 x 50 µl
Anti-P2X7 Receptor     #APR-004     1 x 50 µl
Anti-P2X7 Receptor (extracellular)     #APR-008     1 x 50 µl

 

 

 ● Store-Operated Ca²⁺ Channel Antibody Explorer Kit   

 

Changes in the cytoplasm free Ca²⁺ concentration [Ca²⁺]_in regulate a large number of relevant physiological processes and have a major impact on cell development and function. The identification of STIM, the endoplasmic reticulum (ER) Ca²⁺ sensor, and Orai, a key subunit of the Ca²⁺ release-activated Ca²⁺ (CRAC) channel pore, have provided the tools to illuminate the mechanisms of CRAC channel regulation, that play an important role in modulation of the free Ca²⁺ concentration in non-excitable cells. Store-operated Ca²⁺ entry (SOCE) is a common and ubiquitous mechanism for regulating Ca²⁺ influx into cells and is widely expressed in most tissues.

Alomone Labs is pleased to offer the Store-Operated Ca²⁺ Channel Antibody Explorer Kit (#AK-209). This Explorer Kit includes antibodies targeted against CRAC channels along with their respective peptide control antigen (excludes mouse monoclonal antibodies). An ideal tool for screening purposes.

Compound:

Anti-Human Orai1 (extracellular)     #ACC-060     1 x 50 µl
Anti-Human Orai1 (extracellular)     #ALM-025     1 x 100 µg
Anti-Orai1 (extracellular)     #ACC-062     1 x 50 µl
Anti-Orai2     #ACC-061     1 x 50 µl
Anti-Orai3     #ACC-065     1 x 50 µl
Anti-STIM1 (extracellular)     #ACC-063     1 x 50 µl
Anti-STIM2     #ACC-064     1 x 50 µl
Anti-TRPC1     #ACC-010     1 x 50 µl
Anti-TRPC1 (extracellular)     #ACC-118     1 x 50 µl

 

 

 ● TRPC Channel Antibody Explorer Kit   

 

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.^1-4

The TRPC subfamily consists of seven proteins named TRPC1 to 7, which can be further divided into four subgroups based on their sequence homology and functional similarities:
1.) TRPC1 2.) TRPC4 and TRPC5 3.) TRPC3, TRPC6, TRPC7 4.) TRPC2.^2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.

The TRPC1, TRPC4 and TRPC5 can be activated either by Ca²⁺ store depletion or by GPCR stimulation pathways, while TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. TRPC1, 4 and TRPC5 are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells.⁶

Alomone Labs is pleased to offer the TRPC Channel Antibody Explorer Kit (#AK-212). The Explorer Kit contains TRPC channel antibodies, ideal for screening purposes.

Compound:

Anti-TRPC1     #ACC-010     1 x 50 µl
Anti-TRPC1 (extracellular)     #ACC-118     1 x 50 µl
Anti-TRPC2      #ACC-027     1 x 50 µl
Anti-TRPC3     #ACC-016     1 x 50 µl
Anti-TRPC3     #AGP-018     1 x 50 µl
Anti-TRPC4     #ACC-018     1 x 50 µl
Anti-TRPC4 (extracellular)      #ACC-119    1 x 50 µl

 

 

 ● TRPM Channel Antibody Explorer Kit   

 

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.¹ The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.³

According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.^1-4

The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.⁵

Alomone Labs is pleased to offer the TRPM Channel Antibody Explorer Kit (#AK-210). This Explorer Kit includes antibodies directed to TRPM channels.

Compound:

Anti-Human TRPM1     #ACC-041     1 x 50 µl
Anti-TRPM2     #ACC-043     1 x 50 µl
Anti-TRPM3 (extracellular)     #ACC-050     1 x 50 µl
Anti-TRPM4     #ACC-044     1 x 50 µl
Anti-TRPM5     #ACC-045     1 x 50 µl
Anti-TRPM6 (extracellular)     #ACC-046     1 x 50 µl
Anti-TRPM7     #ACC-047     1 x 50 µl
Anti-TRPM8 (extracellular)     #ACC-049     1 x 50 µl

 

 

 ● TRPV Channel Antibody Explorer Kit   

 

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.¹ The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.³

According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).^1-4 The TRPV subfamily consists of six members, TRPV1-6.⁵

Alomone Labs is pleased to offer the TRPV Channel Antibody Explorer Kit (#AK-211). This Explorer Kit includes antibodies directed to TRPV channels.

Compound:

Anti-TRPV1     #ACC-030      1 x 50 µl
Anti-Rat TRPV1 (extracellular)     #ACC-029     1 x 50 µl

 

 

 ● TTX-Resistant Na_V Channel Antibody Explorer Kit   

 

Voltage-gated sodium channels (Na_v) are essential for the generation of action potentials and for cell excitability.¹ Na_v channels are activated in response to depolarization and selectively allow flow of Na⁺ ions.

To date, nine Na_v α subunits have been cloned and named Na_v1.1-Na_v1.9.^4-5  The Na_v channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Na_v1.1, Na_v1.2, Na_v1.3, Na_v1.4, Na_v1.6 and Na_v1.7)  and TTX-resistant (Na_v1.5, Na_v1.8 and Na_v1.9).^2-3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific.

Alomone Labs is pleased to offer the  TTX-Resistant Na_V Channel Antibody Explorer Kit (#AK-223). This Explorer Kit includes antibodies targeted against voltage-gated Na⁺ channels along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-Na_V1.5     #ASC-005     1 x 50 µl
Anti-Na_V1.5     #AGP-008     1 x 50 µl
Anti-Human Na_V1.5     #ASC-013      1 x 50 µl
Anti-Na_V1.8     #ASC-016     1 x 50 µl
Anti-Na_V1.8     #AGP-029     1 x 50 µl
Anti-Na_V1.9     #ASC-017     1 x 50 µl
Anti-Na_V1.9     #AGP-030     1 x 50 µl
Anti-Pan Na_V     #ASC-003     1 x 50 µl
Anti-Pan Na_V     #AGP-007     1 x 50 µl
Anti-Na_Vβ₁ (extracellular)     #ASC-041     1 x 50 µl
Anti-Na_Vβ₂     #ASC-007     1 x 50 µl
Anti-Na_Vβ₄      #ASC-044     1 x 50 µl

 

 

 ● TTX-Sensitive Na_V Channel Antibody Explorer Kit   

 

Voltage-gated sodium channels (Na_v) are essential for the generation of action potentials and for cell excitability.¹ Na_v channels are activated in response to depolarization and selectively allow flow of Na⁺ ions.

To date, nine Na_v α subunits have been cloned and named Na_v1.1-Na_v1.9.^4-5  The Na_v channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Na_v1.1, Na_v1.2, Na_v1.3, Na_v1.4, Na_v1.6 and Na_v1.7)  and TTX-resistant (Na_v1.5, Na_v1.8 and Na_v1.9).^2-3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific.

Alomone Labs is pleased to offer the TTX-Sensitive Na_V Channel Antibody Explorer Kit (#AK-224). This Explorer Kit includes antibodies targeted against voltage-gated Na⁺ channels along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-Na_V1.1     #ASC-001     1 x 50 µl
Anti-Na_V1.2     #ASC-002     1 x 50 µl
Anti-Na_V1.2     #AGP-026     1 x 50 µl
Anti-Na_V1.3     #ASC-004     1 x 50 µl
Anti-Na_V1.4     #ASC-020     1 x 50 µl
Anti-Na_V1.6     #ASC-009     1 x 50 µl
Anti-Na_V1.7     #ASC-008     1 x 50 µl
Anti-Pan Na_V     #ASC-003     1 x  50 µl
Anti-Pan Na_V     #AGP-007     1 x 50 µl
Anti-Na_vβ₁ (extracellular)     #ASC-041     1 x 50 µl
Anti-Na_vβ₂     #ASC-007     1 x 50 µl
Anti-Na_vβ₄     #ASC-044     1 x 50 µl

 

 

 ● NMDA Receptor Antibody Explorer Kit   

 

The excitatory effects of glutamate (the major excitatory neurotransmitter) are mediated by the activation of ionotropic and metabotropic receptors. These receptors differ in their molecular, biochemical, physiological and pharmacological properties.
The ionotropic glutamate receptors are divided into three main categories: AMPA, NMDA (N-methyl-D-aspartate) and Kainate Receptors. NMDA Receptors have a pivotal role in the regulation of synaptic function in the central nervous system (CNS). They are molecularly composed of three different subunits: NR1, NR2 and NR3 which can undergo alternative splicing.

Alomone Labs is pleased to offer the NMDA Receptor Antibody Explorer Kit (#AK-214). This Explorer Kit includes antibodies targeted against NMDA receptors along with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-NMDA Receptor 1 (GluN1) (extracellular)     #AGC-001     1 x 50 µl
Anti-NMDA Receptor 2A (GluN2A) (extracellular)     #AGC-002     1 x 50 µl
Anti-NMDA Receptor 2B (GluN2B) (extracellular)     #AGC-003     1 x 50 µl
Anti-NMDA Receptor 2C (GluN2C) (extracellular)     #AGC-018     1 x 50 µl
Anti-NMDA Receptor 2D (GluN2D) (extracellular)     #AGC-020     1 x 50 µl
Anti-NMDA Receptor 3A (GluN3A) (extracellular)     #AGC-030     1 x 50 µl
Anti-NMDA Receptor 3B (GluN3B) (extracellular)     #AGC-031     1 x 50 µl

 

 

 ● L-Type Ca_V Channel Antibody Explorer Kit   

 

Voltage-gated Ca²⁺ channels (Ca_V), enable the passage of Ca²⁺ ions in a voltage dependent manner. These heteromeric entities are formed in part by the pore-forming α1 subunit which determines the biophysical and pharmacological properties of the channel¹.

L-type Ca²⁺ channels make up one of three voltage-gated Ca²⁺ channel families. Four different α1 isoforms (Ca_V1.1 to Ca_V1.4) belong to the L-type subfamily. Structurally, each α1 subunit has four homologous domains (I-IV) and each domain has a six transmembrane section. Like many other voltage-gated channels, L-type Ca²⁺ channels have auxiliary subunits which are responsible for modulating the surface expression and properties of the channels^2-5.

Ca_V1.1 is mostly expressed in the skeletal muscle, while Ca_V1.4 is mainly detected in the retina. The expression of both Ca_V1.2 and Ca_V1.3 is more extensive and includes neurons, heart, smooth muscle, inner ear, retina and pancreas⁶. L-type Ca²⁺ channels are involved in and modulate a variety of physiological functions such as muscle contraction, hormone secretion, neuronal excitability and gene expression⁵.

Alomone Labs is pleased to offer the L-Type Ca_V Channel Antibody Explorer Kit (#AK-215). The Explorer Kit contains L-type channel antibodies with their respective peptide control antigen. An ideal tool for screening purposes.

Compound:

Anti-Ca_V1.2     #ACC-003     1 x 50 µl
Anti-Ca_V1.2     #AGP-001     1 x 0.1 ml
Anti-Human Ca_V1.2     #ACC-022     1 x 50 µl
Anti-Ca_V1.2a     #ACC-013     1 x 50 µl
Anti-Ca_V1.3     #ACC-005     1 x 50 µl
Anti-Ca_V1.3 (extracellular)     #ACC-311     1 x 50 µl
Anti-Ca_Vα2δ1     #ACC-015     1 x 50 µl
Anti-Ca_vα2δ2 (extracellular)     #ACC-102     1 x 50 µl
Anti-Ca_vα2δ3 (extracellular)     #ACC-103     1 x 50 µl
Anti-Ca_vα2δ4 (extracellular)     #ACC-104     1 x 50 µl
Anti-Ca_Vβ₁     #ACC-106     1 x 50 µl
Anti-Ca_Vβ2     #ACC-105     1 x 50 µl
Anti-Ca_Vβ₃     #ACC-008     1 x 50 µl
Anti-Ca_Vγ₂     #ACC-012     1 x 50 µl
Anti-Ca_Vγ3     #ACC-113     1 x 50 µl
Anti-Ca_Vγ₄     #ACC-114     1 x 50 µl
Anti-Ca_Vγ5     #ACC-115    1 x 50 µl

 

 

 ● Non-L-Type Ca_V Channel Antibody Explorer Kit   

 

Voltage-dependent Ca²⁺ channels (Ca_v) form an important route for Ca²⁺ entry into cells, upon deviations from the cells’ resting membrane potential. There are ten Ca_v α₁ subunit genes, divided into three subfamilies on both a functional and sequence homology basis. The Ca_v1 subfamily includes four genes which encode the L-type channels, the Ca_v3 subfamily includes three genes encoding the T-type channels and the Ca_v2 subfamily consists of three isoforms encoding P/Q- , N- and R-type channels, with all three contributing to neurotransmitter release in neurons.

Alomone Labs is pleased to offer the Non-L-Type Ca_V Channel Antibody Explorer Kit (#AK-216). The Explorer Kit contains non-L-type Ca_V channel antibodies, ideal for screening purposes.

Compound:

Anti-Ca_V2.1     #ACC-001     1 x 50 µl
Anti-Ca_V2.2     #ACC-002     1 x 50 µl
Anti-Ca_V2.3     #ACC-006     1 x 50 µl
Anti-Ca_V3.1     #ACC-021     1 x 50 µl
Anti-Ca_V3.2     #ACC-025     1 x 50 µl
Anti-Ca_V3.3     #ACC-009     1 x 50 µl
Anti-Ca_Vpan α1     #ACC-004     1 x 50 µl
Anti-Ca_Vα2δ1     #ACC-015     1 x 50 µl
Anti-Ca_vα2δ2 (extracellular)     #ACC-102     1 x 50 µl
Anti-Ca_vα2δ3 (extracellular)     #ACC-103     1 x 50 µl
Anti-Ca_vα2δ4 (extracellular)     #ACC-104     1 x 50 µl
Anti-Ca_Vβ1     #ACC-106     1 x 50 µl
Anti-Ca_Vβ2     #ACC-105     1 x 50 µl
Anti-Ca_Vβ₃     #ACC-008      1 x 50 µl
Anti-Ca_Vγ2     #ACC-012     1 x 50 µl
Anti-Ca_Vγ4     #ACC-114     1 x 50 µl
Anti-Ca_Vγ5     #ACC-115     1 x 50 µl

  

 

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