The DetectX Prostaglandin E2 (PGE2) Multi-Format Enzyme Immunoassay kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, tissues, saliva and culture media samples.  This one EIA kit allows all samples to be measured with just one kit.  The monoclonal antibody has super-fast kinetics and shows the same binding characteristics at 2 or 16 hour incubation.  The kit allows samples as low as 2 pg/mL to be measured on less than 3 hours. It exhibits excellent cross reactivity and low sample requirements.

A PGE2 standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A PGE2-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to PGE2. After either an incubation the plate is washed and substrate is added. The substrate reacts with the bound PGE2-peroxidase conjugate. After a 30 minute incubation the intensity of the generated color is read at 450nm.

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