Biotium社 Direct SARS-CoV-2 detection using an EvaGreen-based RT-LAMP assay
RT-LAMP assays using in-house produced enzymes show promise for accurate and affordable SARS-CoV-2 testing
In the efforts to limit the spread of SARS-CoV-2, extensive testing has played a crucial role. However, traditional reverse transcription PCR (RT-PCR) testing requires specialized equipment and reagents that are difficult to apply on a large scale. A more accessible form of PCR known as reverse transcription loop-mediated isothermal amplification (RT-LAMP), has been developed, but distribution of molecular biology reagents remains limited.
In a recent publication in Nature Scientific Reports, A. Alekseenko, et al. developed a set of protocols for production of DNA polymerases that are simple to make and ideal for use in RT-LAMP-based detection of SARS-CoV-2. The authors performed RT-LAMP assays to compare the performance of the in-house produced enzymes with commercially available alternatives. They used EvaGreen® Dye to track DNA amplified from synthetic SARS-CoV-2 RNA templates. Results show EvaGreen® Dye provided a higher dynamic range of fluorescence than SYBR® Green I during...
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