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Biotium社 Microbes under the microscope?



Whether you're studying bacteria, yeast, or other microbes, Biotium offers a vast array of tools and solutions for your research needs.


Explore our microbiology products below:

Bacterial Stains



Heat-killed Pseudomonas and Micrococcus stained with the Bacterial Viability and Gram Stain Kit. CF®488A WGA gram-positive Micrococcus (green), EthD-III stained dead cells (red), and DAPI (blue).


E. coli stained with BactoView™ Live Green (stains DNA) and SynaptoRed™ (stains the membrane).                           

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Yeast and Fungi Stains

  • ViaVac™ Red/Green is a yeast-specific vitality dye that stains metabolically active cells
  • Live-or-Dye™ fixable dead cell stains for microscopy and flow cytometry, available in 14 colors
  • Yeast Vitality Staining Kits for co-staining of cell walls and metabolically active cells
  • Fixable cell membrane stains with green, red, and far-red options
  • Organelle specific stains for yeast vacuoles, mitochondria, nuclei, and more
Image(left).jpgS. cerevisiae stained with the Yeast Vitality Staining Kit: Calcofluor White (blue) and ViaVac™ stains vacuoles of metabolically active cells red and the cytoplasm of all cells green (not shown). Image(right).jpgS. cerevisiae stained with the Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit: Thiazole Orange stains all cells (green) and Live-or-Dye™ 568/583 to stain dead cells (red, appears yellow in overlay).
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Still culturing for viability? Upgrade to Viability PCR

Viability PCR (v-PCR) is a powerful method for sensitive and rapid detection of viable microbes without the need for culturing. 

  • Determine percent viable cells using viability PCR with PMA & PMAxx™
  • Superior speed, sensitivity, and accuracy over culture-based viability methods
  • Works even in complex samples or mixed cultures
  • Strain-specific starter kits available for E. coli, S. enterica, S. aureus, L. monocytogenes, and more
  • Works with viable but non-culturable bacteria (VBNC) 

Image(large).jpgViability PCR dyes like PMAxx™ or PMA are membrane-impermeant, which makes them dead cell specific. Once inside of a dead cell, they bind to DNA. Exposure to intense visible light renders the dyes reactive, and causes them to covalently attach to the DNA. This DNA modification prevents amplification in subsequent PCR reactions.

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Related Technologies

Immunofluorescence Microscopy 
PCR & DNA Amplification
Protein Gel Stains

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