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Biotium社 Move Past Annexin V -- Try NucView® for Real-Time Apoptosis Monitoring.




Monitor apoptosis in real-time by flow cytometry or microscopy with NucView® Caspase-3 Substrates. Validated in hundreds of publications and over a hundred cell types, NucView® substrates are a gold standard for directly detecting caspase-3/7 activity in live cells.

  • Simple: No-wash, homogeneous assay for real-time or end-point apoptosis detection in live cells
  • Fast and Fixable: Formaldehyde fixable staining in only 15 minutes
  • Non-toxic: Useful for long multi-day experiments
  • Choice: Available with blue, green, or red fluorescence
  • Validated: NucView® 488 is validated in more than 100 cell types and 200 publications. Check out our full list of references and validated strains.


How It Works

When introduced to cells, the NucView® substrate is initially non-fluorescent and is able to penetrate the plasma membrane and enter the cytoplasm. During apoptosis, caspase-3/7 cleaves the substrate and releases the high-affinity DNA dye which leads to nuclear fluorescent staining that can be monitored in real-time.




Interested in learning more?

Download our E-Book “Visualizing Apoptosis as it Happens” to learn about the intrinsic and extrinsic apoptosis pathways, the morphological stages, and a wide range of assays useful in detecting early or late events.



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