Biotium社 Proteins: Detect, Quantitate, and Analyze
One-Step Blue® & One-Step Lumitein™
Rapid, Non-toxic Protein Gel Stains
Biotium's One-Step protein stains are safe and sensitive alternatives for colorimetric, UV, or fluorescent detection.
- Non-toxic: Aqueous formulations; certified under CCR Title 22 as non-hazardous for safe and easy disposal
- Rapid: Quick 5 to 60 minute staining in a single step without fixation or washing
- Clearer: One-Step Blue® provides clearer staining than Coommassie® or other colorimetric stains
- Versatile: Compatible with Edman-based sequencing and mass spectrometry
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Unstained protein ladder was loaded on a 4-12% Bis-Tris gel and stained for 1 hour with Coomassie Brilliant Blue R-250 (CBB), Simply Blue™ SafeStain (SB), and One-Step Blue® (OSB) following SDS-PAGE.
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GloMelt™ Protein Thermal Stability Assay
A versatile thermal shift assay for analyzing protein unfolding or thermal stability.
- Green fluorescence optimal for qPCR instruments and ROX normalization
- Compatible with a wide pH range, high detergent, reducing agents, and common buffers/excipients
- Great for high throughput assays, low reaction volumes, and low protein concentrations
- Highly soluble and stable in aqueous buffers
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Environmentally sensitive fluorescent dyes can be used to monitor the temperature dependent unfolding of a protein. The protein’s melting temperature (Tm) is a reporter of the protein’s thermal stability.
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AccuOrange™ Fluorescent Protein Quantitation Assay
Highly sensitive and versatile fluorescence-based assay for quantifying purified protein samples.
- More sensitive than BCA, Bradford, and Lowry assays
- Excellent linearity and low variability
- Stable fluorescence signal
- Compatible with reducing agents and other additives
- For use with purified protein or antibody samples
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The AccuOrange™ fluorescent protein quantitation assay is more linear and less variable than the NanoOrange® assay.
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