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biotium社 Studying bacteria, yeast, or other microbes? We've got a stain or assay for that!

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Whether you're studying bacteria, yeast, or other microbes, Biotium offers a vast array of tools and solutions for your research needs.

Explore our microbiology products below:

Bacterial Stains

  • BactoView™ Live Stains are green and red nucleic acid binding dyes that stain all bacteria. 
  • WGA CF® Dye conjugates for rapid and sensitive gram staining, available in 13 colors.
  • Bacterial Viability and Gram Stain Kit for co-staining dead cells and gram-positive cells.
  • Fixable cell membrane stains with green, red, and far-red options.
  • Classic and novel nuclear stains available from blue to far-red.

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Heat-killed Pseudomonas and Micrococcus stained with the Bacterial Viability and Gram Stain Kit. CF®488A WGA gram-positive Micrococcus (green), EthD-III stained dead cells (red), and DAPI (blue).

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E. coli stained with BactoView™ Live Green (stains DNA) and SynaptoRed™ (stains the membrane).

Yeast and Fungi Stains

  • ViaVac™ Red/Green is a yeast-specific vitality dye that stains metabolically active cells.
  • Live-or-Dye™ fixable dead cell stains for microscopy and flow cytometry, available in 12 colors.
  • Yeast Vitality Staining Kits for co-staining of cell walls and metabolically active cells.
  • Organelle specific stains for yeast vacuoles, mitochondria, cell wall, nucleus, and more.

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S. cerevisiae stained with the Yeast Vitality Staining Kit: Calcofluor White (blue) and ViaVac™ stains vacuoles of metabolically active cells red and the cytoplasm of all cells green (not shown).

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S. cerevisiae stained with the Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit: Thiazole Orange stains all cells (green) and Live-or-Dye™ 568/583 to stain dead cells (red, appears yellow in overlay).

Still culturing for viability? Upgrade to Viability PCR

Viability PCR (v-PCR) is a powerful method for sensitive and rapid detection of viable microbes without the need for culturing. 

  • PMA & PMAxx™ dyes selectively inhibit PCR amplification of DNA from dead cells.
  • Superior speed, sensitivity, and accuracy over culture-based viability methods.
  • Works even in complex samples or mixed cultures.
  • Able to detect viable but non-culturable bacteria (VBNC).

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Viability PCR dyes like PMAxx™ or PMA are membrane-impermeant, which makes them dead cell specific. Once inside of a dead cell, they bind to DNA. Exposure to intense visible light renders the dyes reactive, and causes them to covalently attach to the DNA. This DNA modification prevents amplification in subsequent PCR reactions.

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