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Cytoskeleton社 F-actin蛍光染色試薬 Acti-stain™ Fluorescent Phalloidins

Acti-stain™シリーズは、F-actin(アクチン)の検出に用いられるPhalloidin(ファロイジン)に、高輝度かつ退色耐久性に優れた色素をコンジュゲートした製品です。 

 

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メーカー情報 

 

The Acti-stain™ line of fluorescent phalloidins has been developed with an emphasis on creating exceptionally bright and stable probes at an economical price. Side-by-side comparisons with leading competing products demonstrate that you will enjoy savings while not sacrificing the quality of the stain when switching to an Acti-stain™ probe. Additionally, these probes are compatible with popular filter sets such as FITC, TRITC and Cy5. The combination of in-house manufacturing, stringent quality control and convenient packaging provides a great value. Give them a try and see for yourself.

 

Product Uses Include

 

Actin staining is very useful in determining the structure and function of the cytoskeleton in living and fixed cells. The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures.

 

■ Biochemical characteristics of fluorescent phalloidins

Acti-stain.jpg 

 

  Acti-stain™ 488   

 

PHDG1_img1_1.jpg PHDG1_scan.jpg
Swiss 3T3 cell stained with anti-vinculin (red), Dapi (blue nucleus) and F-actin is stained with Acti-stain™ 488 (green F-actin, Cat.# PHDG1). Absorbance and fluorescence scan of Acti-stain™ 488. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 350-750 and 500-750 nm, respectively. Absorbance peaks at 500 nm and fluorescence at 550 nm.

 

 
  Acti-stain™ 555   

 

PHDH1_img1.jpg PHDH1_scan.jpg
Swiss 3T3 cell with activated Rho, nucleus is stained with Dapi (blue) and F-actin is stained with Acti-stain™ 555 (red F-actin, Cat.# PHDH1). Absorbance and fluorescence scan of Acti-stain™ 555. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 300-750 and 560-750 nm, respectively. Absorbance peaks at 560 nm and fluorescence at 575 nm.
 

 

  Acti-stain™ 670                                           

 

PHDN1_img1.jpg PHDN1_scan.jpg
Mitotic Swiss 3T3 cell, F-actin stained with Acti-stain™ 670 (far-red, Cat. # PHDN1), nuclear DNA stained with Dapi (blue). Absorbance and fluorescence scan of Acti-stain™ 670. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 300-750 and 600-750 nm, respectively. Absorbance peaks at 625 nm and fluorescence at 675 nm.
 

詳細情報 >> Cytoskeleton社WEBサイト

 

 

この製品に関するご意見・ご照会・お問合せはこちら  

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