Fount of Information

Cytoskeleton社 Active RhoA Detection - Cited, Trusted Results




Home Products Distributors News


RhoA G-LISA Activation Assay Kit


The RhoA G-LISA™ Activation Assay is faster and more precise than traditional pull-down methods and is a trusted approach to measure Active RhoA.


Why Use RhoA G-LISA (BK124)?

The G-LISA RhoA activation assays are ELISA based Rho activation assays with which you can measure RhoA activity in cells in less than 3 h.


• High Sensitivity and Accuracy

• Small Amount of Material Required

• Compatible with Tissue and Cell Lysates


Product Uses for BK124 Include:


  • Rho signaling pathway studies
  • Studies of Rho activators and inactivators
  • Rho activation assays with limited material



RhoA activation by calpeptin measured by G-LISA kit BK124. Swiss 3T3 (mouse) cells were serum starved for 24 h and treated with calpeptin (Cal; 0.1 mg/ml for 30 min) or DMSO carrier only (SS). 10 µg of cell lysates were subjected to the G-LISA™ assay. Absorbance was read at 490 nm.





Recent Citations


Nath A. et al. Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses. Cell Rep. 2022 Mar 1;38(9):110433.


Swaminathan B. et al. Endothelial Notch signaling directly regulates the small GTPase RND1 to facilitate Notch suppression of endothelial migration. Sci Rep. 2022 Jan 31;12(1):1655. doi: 10.1038/s41598-022-05666-1.


Tabdanov E. et al. Engineering T cells to enhance 3D migration through structurally and mechanically complex tumor microenvironments. Nat Commun. 2021 May 14;12(1):2815. doi: 10.1038/s41467-021-22985-5.



Cytoskeleton Inc's Activation Assay News


News.jpg ECM_Stiffness.gif
Oncogenic RAS drives cells to the cancerous state through a wide range of mechanisms, including: the establishment of cancer cell plasticity (1), enhancement of cell migration and metastasis (2) and metabolic reprogramming towards anabolic metabolism (3). This Newsletter focuses on the emerging area of RAS driven oncogenesis via the evasion of host immune attack...... Vascular smooth muscle cells (VSMCs) oscillate between a differentiated (contractile) and a de-differentiated (synthetic) phenotype. In a recent study by Talwar et al., the group discovered that VSMCs, under selective ECM conditions, can also exist in a null state and can be driven towards the conventional differentiated or de-differentiated phenotypes in response to Rac and Rho signaling......
Read_the_NewsLetter.PNG Click_Here.PNG

Cytoskeleton has written quite a bit about Small G-Proteins and Activation Assays over the years, and we've collected our newsletters in one convenient place that you can review at your leisure.






Actin_Tools.gif Tubulin_Tools.gif Small_G-protein_Tools.gif
Actin Tools Tubulin Tools Small G-Protein Tools

Fount of Information は、新商品、新規取扱メーカーなどの情報をいち早く紹介するコンテンツです。情報発信のスピードを重視しているコンテンツのため、現時点で法規制や取り扱いを確認できていない商品、定価を設定できていない商品があります。ご要望やご照会を受けた商品について、法令整備や在庫の充実を図ります。