Cytoskeleton社 ECM Tools: Purified and Labeled Laminin

Laminin the best known member of a family of basement membrane glycoproteins that play a role in cell adhesion, migration, growth and differentiation. Laminins also promote neurite outgrowth and regeneration.

Features of Rhodamine Fibronectin (LMN02):

Laminin-1 is purified from EHS tumor tissue.
The laminin is >90% pure (Figure).
The protein is modified to contain covalently linked HiLyte 488TM dyes at random surface lysines.
Final labeling stoichiometry is 2-5 dyes per protein molecule.
The material is guaranteed to contain <15% of free dye and >85% of dye conjugated to laminin.
HiLyte 488TM laminin can be detected using a filter set of 502nm excitation and 527nm emission.

Product Uses Include:

Cell invasion assays
FACS analysis of laminin binding cells

Legend: 20 µg of unlabeled laminin (Lane 1) and 20 µg of HiLyte 488TM laminin (Lane 2) was separated by electrophoresis in a 4-20% SDS-PAGE system. The unlabeled protein was stained with Coomassie Blue and visualized in white light. The labeled protein was visualized under UV light. The alpha subunit runs at 400 kDa (top band) while the beta and gamma subunits run as a 225 kDa doublet (lower band). Arrows indicate unincorporated dye. In this example unincorporated dye = 13%.

Cytoskeleton's Full Selection of ECM Proteins

The Extracellular Matrix (ECM) is composed of collagen, non-collagenous glycoproteins, and proteoglycans. These components are secreted from cells to create an ECM meshwork that surrounds cells and tissues. The ECM regulates many aspects of cellular function, including the cells' dynamic behavior, cytoskeletal organization, and intercellular comm-unication.

Click on the above links to learn more about Cytoskeleton's ECM products.

Related Tool - Protein Assay Solution (Cat. # ADV02)

Developed by scientists at Cytoskeleton, the Precision Red Advanced Protein Assay (cat. # ADV02) is highly recommended for measuring purified protein and total cell protein alike.

ADV02 is compatible with detergents, and retains the same similarity between different purified proteins. In comparison to the Bradford assay these assays read different purified proteins with two fold greater accuracy which allows the user to rely on the simple A = Ecl formula to convert the OD into a real protein concentration.

Figure 1: Standard curves of Actin, BSA, IgG and Tubulin protein using the Precision Red Advanced Protein Assay Reagent

www.cytoskeleton.com/ecm

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