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Cytoskeleton社 Effective Active Ras Detection With Cytoskeleton's Activation Assay




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Active Ras Small G-Protein Analysis with Trusted Activation Assay Tools


 The Ras small G-proteins act as molecular switches that transmit cellular signals through an array of effector proteins. Ras plays an important role in many cellular functions including the control of cell proliferation and differentiation. The Ras switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of the GTPases is of obvious biological significance and is a subject of intense investigation.


Ras Pull-Down Activation Assay Biochem Kit (Cat. # BK008) Components:

  • GST tagged Raf1-RBD protein bound to colored glutathione agarose beads (Cat. # RF02).
  • Ras monoclonal antibody
  • Cell lysis Buffer
  • Protease inhibitor cocktail (Cat. # PIC02)
  • Wash Buffer
  • Loading Buffer
  • STOP Buffer
  • GTPγS (non-hydrolyzable GTP analog, Cat. # BS01)
  • GDP



Raf-RBD_bead_pulldown_Assays.jpgFigure 1. Raf-RBD bead pulldown Assays. A. Extract (300 μg) from human platelet cells was loaded with GTPgammaS (GTP lanes 1 & 2) or GDP (GDP lanes 3 & 4) using the method described in the manual. Lane 5 shows 30 ng of recombinant His-Ras control protein. 



Selected BK008 Citations


Tulpule A et al. Kinase-mediated RAS signaling via membraneless cytoplasmic protein granules. Cell. 2021 May 13;184(10):2649-2664.e18. doi: 10.1016/j.cell.2021.03.031.


Kirolos S et al. A chemorepellent inhibits local Ras activation to inhibit pseudopod formation to bias cell movement away from the chemorepellent. Mol Biol Cell . 2022 Jan 1;33(1):ar9. doi: 10.1091/mbc.E20-10-0656. Epub 2021 Nov 17. 



Check out this recent Newsletter focused on small g-proteins


Rac1 And Cdc42 Pathways As Pivotal Axis In Future Metastatic Cancer Therapy

The appearance of Rac and Cdc42 proteins on the radar of the medical scientific community started in the 1990’s with seminal papers by Professor Alan Hall’s laboratory at MRC, UK. They identified Rac and Cdc42 small G-proteins (R&C) as central regulators of cell motility, shape and protrusions (lamellipodia and filopodia)1,2. Now several decades later, the concept has been fully proven in a multitude of publications (see refs. in these reviews 3 & 4), and the realization that these proteins are central to cancer cell motility and invasion has led the field to explore them as candidates for therapeutic intervention.....


Read More



Take a Peek at Other Cytoskeleton's Activation Assays


Cytoskeleton's Small G-protein Activation Assays measure the GTP-bound form of the protein from a cell or tissue extract. 

  • Activation assays are available in two formats: the traditional pull-down bead format and our advanced ELISA based G-LISA format. 
  • MeasurArf1Cdc42RacRalRas, and Rho
  • Accurate, sensitive, and user-friendly formats
  • The most trusted and cited activation assays available

Watch our video or browse the options below to learn more about which format is right for you.









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Actin Tools Tubulin Tools Small G-Protein Tools

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