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Ras G-LISA Activation Assay Kit

Effective Analysis of Active Ras

Why Use Ras G-LISA Activation Assay Kit (BK131)?

The Ras G-LISA® kit contains a Ras GTP-binding protein linked to the wells of a 96 well plate. Active, GTP-bound Ras in cell/tissue lysates will bind to the wells while inactive GDP-bound Ras is removed during washing steps. The bound active Ras is detected with a Ras specific antibody. The degree of Ras activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.

Product Uses for BK131 Include:

• Ras signaling pathway studies
• Ras activation assays with primary cells
• Studies of Ras activators and inactivators
• Ras activation assays with limited material
• High throughput screens for Ras activation

Above: Ras activation by EGF measured by G-LISA™.  HeLa cells were serum starved (SS) for 24 h and treated with EGF (100 ng/ml for 2 min). 25, 12.5, 5, 1.25 µg of cell lysates were subjected to the G-LISA™ assay. Absorbance was read at 490 nm. Data are background subtracted

Recent Ras GLISA Kit Citations

Nataraj, Nishanth Belugali et al. Nucleoporin-93 reveals a common feature of aggressive breast cancers: robust nucleocytoplasmic transport of transcription factors Cell Reports 2022 ISSN 2211-1247

Swaminathan, Bhairavi et al. Endothelial Notch signaling directly regulates the small GTPase RND1 to facilitate Notch suppression of endothelial migration Scientific Reports 2022 ISSN 2045-2322

Hofmann, Marco H. et al. Bi-3406, a potent and selective sos1–kras interaction inhibitor, is effective in kras-driven cancers through combined mek inhibition Cancer Discovery 2021 ISSN 2159-8290

Recent Small-G Protein Newsletter and Citation Spotlight

Vascular smooth muscle cells (VSMCs) oscillate between a differentiated (contractile) and a de-differentiated (synthetic) phenotype. In a recent study by Talwar et al., the group discovered that VSMCs, under selective ECM conditions, can also exist in a null state and can be driven towards the conventional differentiated or de-differentiated phenotypes in response to Rac and Rho signaling......

The small G-protein RhoA, functions as a molecular switch through interactions with GTP and GDP, to regulate an array of cellular processes including cell proliferation, cell shape, and cytoskeletal regulation. Seminal studies by Ridley and Hall showed that RhoA was activated by serum stimulation[1]; additionally, it is now appreciated that RhoA is a critical signaling mediator for some G protein-coupled receptors (GPCRs).