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Cytoskeleton社 Ubiquitin Enrichment With UBA01




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Detect Endogenous Ubiquitination Of Your Target Proteins


Ubiquitination (Ub) of target proteins have been shown to have profound effects, including protein degradation, localization, and function. Ubiquitin affinity beads (Cat. # UBA01) have been optimized in order to detect endogenous levels of mono- and poly-ubiqutinated proteins. The affinity moeities have been cross-linked to beads for low leaching and cleaner detection of the protein of interest.


Signal-Seeker™ Ubiquitin Affinity Beads (Cat. # UBA01)


Product Uses Include

  • Investigate transient regulatory Ubiquitin mechanisms


  • Measure Ub signalling events of target proteins 


  • Discover new Ub modifications of your protein of interest




Also available as a comprehensive kit, which is great for investigators new to studying ubiquitinated proteins.  Check out Cytoskeleton BK161 Ubiqutination Detection Kit



Legend: Swiss 3T3 cells were pre-treated with MG-132, then either untreated or treated with CNF1 (CN04) for 3 hours prior to lysis with BlastR buffer. The BK161 kit was utilized to perform the IP on 300 μg of lysate per condition. Lane 1: 3 μg input untreated lysate, Lane 2: Ubiquitin Affinity beads (UBA01) plus untreated lysate, Lane 3: UBA01 plus CN04 treated lysate, Lane 4: ubiquitin control beads (CUB02) plus untreated lysate, Lane 5: CUB02 plus CN04 treated lysate. Samples were analyzed for Rac1 ubiquitination using an anti-Rac1 antibody.


Check Out This Recent Publication


Kha The Nguyen et. al. The MARCHF6 E3 ubiquitin ligase acts as an NADPH sensor for the regulation of ferroptosis.  Nat Cell Biol . 2022 Aug;24(8):1239-1251. doi: 10.1038/s41556-022-00973-1.




Selected Signal-Seeker Reference Material



Cytoskeleton has prepared FAQs, Background Information, a PTM eBook, and Tech Tips specific to PTMs. Explore the links below to learn more!



Video LibraryTech Tips and FAQs - eBook - PTM Newsletters - Tips For New Investigators


Signal-Seeker Detection Kits



Signal-Seeker™ Ubiquitination Detection Kits (30 Assays) (Cat. # BK161)


Signal-Seeker™ Ubiquitination Detection Kits (10 Assays) (Cat. # BK161-S


Signal-Seeker™ Acetylation Detection Kits (30 Assays) (Cat. # BK163)


Signal-Seeker™ Acetylation Detection Kits (10 Assays) (Cat. # BK163-S


Signal-Seeker™ Phosphotyrosine Detection Kits (30 Assays) (Cat. # BK160)


Signal-Seeker™ Phosphotyrosine Detection Kits (10 Assays) (Cat. # BK160-S


Signal-Seeker™ SUMOylation 1 Detection Kits (30 Assays) (Cat. # BK165)


Signal-Seeker™ SUMOylation 1 Detection Kits (10 Assays) (Cat. # BK165-S


Signal-Seeker™ SUMOylation 2/3 Detection Kits (30 Assays) (Cat. # BK162)


Signal-Seeker™ SUMOylation 2/3 Detection Kits (10 Assays) (Cat. # BK162-S)



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