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Cytoskeleton社 Unique Reagents: Tropomyosin/Troponin Complex

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Tropomyosin/Troponin Complex

The Tropomyosin / Troponin protein (TT) complex has been purified from bovine cardiac muscle (1). The TT complex is composed of five proteins Tropomyosin alpha: Tropomyosin beta: Troponin C : Troponin I : Troponin T in a stoichiometric ratio of 1:1:1:1:1. The complex has been determined to be biologically active in an F-actin / calcium activated myosin ATPase assay.

Product Uses of Tropomyosin/Troponin Complex (Cat # CS-TT05)

·     Measurement of calcium activated myosin ATPase activity when bound to thin filaments.

 

·     Identification/characterization of proteins or small molecules that affect the TT complex regulation and myosin ATPaseactivity

 

·     Identification/characterization of proteins or small molecules that affect myosin / F-actin interaction

 Figure 2a_2b.gif Figure 2. Tromomyosin / Troponin TCI Complex Protein Purity Determination.

Tropomyosin / Troponin TCI Complex Protein Purity Determination.

 

Figure 2a: A 20 μg sample of TT complex protein was separated by electrophoresis in a 4-20% SDS-PAGE system and stained with Coomassie Blue. Arrow 1 indicates Troponin T (38 kDal), arrow 2 indicates Tropomyosin(alpha+beta, 32 kDal), arrow 3 indicates Troponin I (24 kDal) and arrow 4 indicates Troponin C (18 kDal).

 

Figure 2b: A 2 μg sample of TT complex protein was separated by electrophoresis in a 4-20% SDS-PAGE system, blotted onto PVDF membrane and probed with component specific monoclonal antibodies. Lane 3: Tropomyosin(alpha+beta, 32 kDal, MAb clone:TM311), Lane 4: Troponin T (38 kDal, MAb clone JLT-12 from Sigma) (antibody gave 24 kDal signal—cross reacts with Troponin I), Lane 5: Troponin I (24 kDal, MAb clone MAB1691), and Lane 6: Troponin C (18 kDal, MAb clone 7B9).

 

Protein quantitation was performed using the Precision Red™ Protein Assay Reagent (Cat.# ADV02). See Blue molecular weight markers are from Life Technologies Inc.

 

Other Popular Proteins Developed By Cytoskeleton's

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Cardiac Thin Filament Complex

 Product Uses of Cardiac Thin Filament Complex (CTFC) (CAT # TFC01):

·     Measurement of calcium activated myosin ATPase activity when bound to thin filaments.

 

·    Identification/characterization of proteins or small molecules that affect the CTFC regulation and myosin ATPase activity

·    Identification/characterization of proteins or small molecules that affect myosin / F-actin interaction

Cardoac_Image.jpgAbove: The cardiac thin filament complex (CTFC)  

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Cytoskeleton, Inc. has been a reliable source of compound screening services in the areas of pre-clinical drug development programs and early compound screening in primary HTS projects, as well as secondary screening and compound target validation. We also have extensive experience in gene design and expression with an eye for producing highly purified, biological active proteins.

Our Specialties Include

 

·     Compound Screening Modules

·     Gene Cloning and Protein Purification Modules

·     Assay Development Modules

·     ELISA and Antibody Development

Contact us at tservice@cytoskeleton.com with any questions or requests. We have happy clients from Astra-Zeneca to Pfizer and would love to help speed along your drug development.

 

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