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Cytokine ELISA kits

LumiKine^™ is InvivoGen’s new immunoassay for rapid, sensitive and specific detection of cytokines. By replacing the absorbance-based enzymatic detection reaction of a standard ELISA with its lightning-fast Lucia luciferase/QUANTI-Luc^™ bioluminescence detection system, InvivoGen has created an assay that can save you precious time in the laboratory. Unlike many ELISA kits, which are based on E. coli-produced cytokines, LumiKine™ has been designed using cytokines produced in mammalian cells, for accurate detection of species-specific mammalian cytokines.
LumiKine^™ kits are currently available for human and mouse type I interferons, as well as IL-1α and IL-1β. They allow you to detect the cytokine in a broad concentration range that makes quantification and calibration curves a snap.

• Rapid - Optimized to reduce assay time - With LumiKine^™ Xpress kits, save at least 1 hour compared to standard ELISAs
• Specific - Antibodies raised against natural cytokines by DNA immunization
• Precise - Wide concentration range
• Economical - Each kit contains enough reagents to run five ELISA plates

 

 

 

LumiKine^™ kits 

Four step bioluminescent ELISA kits

LumiKine™ kits are bioluminescent ELISA kits designed to quantify the levels of cytokines in cell culture supernatant, serum and plasma samples using the classical “sandwich” ELISA method.

LumiKine™  kits include a capture antibody, a biotinylated secondary antibody and a streptavidin-Lucia conjugate for detection. This conjugate consists of a streptavidin peptide fused to Lucia luciferase, a small secreted coelenterazine-utilizing luciferase with strong and stable bioluminescence properties. After addition of QUANTI-Luc™, a solution that contains the Lucia luciferase substrate,a bioluminescent signal is  instantaneously emitted. This signal is directly proportional to the concentration of the cytokine produced and quantifiable by a luminometer. The streptavidin-Lucia conjugate can be replaced by the streptavidin conjugate of your choice.

 

 

  

Principle

The monoclonal hIFN-α Capture Antibody is coated onto the wells of a white immuno 96-well plate.

Samples and the hIFN-α Standard are pipetted into these wells, followed by the addition of the monoclonal Biotinylated Antibody (detection antibody).

During the first incubation, the hIFN-α antigen binds to the immobilized hIFN-α Capture Antibody on one site, and during the second incubation, to the Biotinylated Antibody on a second site.

After removal of the excess detection antibody, Streptavidin-Lucia is added. This streptavidin conjugate binds to the Biotinylated Antibody.

After a short third incubation and washing to remove all the unbound Lucia luciferase, QUANTI-Luc™, a solution that contains the substrate of Lucia luciferase, is added. No further incubation is required. The bioluminescent signal is emitted instantaneously and measured in Relative Light Units (RLU) by a luminometer. The intensity of this signal is directly proportional to the concentration of hIFN-α present in the samples.

 

 

 

 

 

 

 

 

 

LumiKine^™ Xpress kits

Three step bioluminescent ELISA kits

LumiKine™ Xpress kits are bioluminescent ELISA kits designed to quantify the levels of cytokines in cell culture supernatant, serum and plasma samples, more rapidly than classical “sandwich” ELISA kits (at least 1 hour).

In the LumiKine™ Xpress kits, the biotinylated secondary antibody and the streptavidin-Lucia conjugate are replaced by a Lucia-conjugated secondary antibody, eliminating an incubation step for faster detection. Lucia luciferase is a small secreted coelenterazine-utilizing luciferase with strong and stable bioluminescence properties.

The levels of cytokines are determined by measuring the luminescence instantaneously produced by Lucia luciferase after hydrolysis of its substrate provided in QUANTI-Luc™. The bioluminescent signal is assessed by luminometry. 

 

 

 

Principle

The hIFN-α Capture Antibody is coated onto the wells of a white immuno 96-well plate.

Samples and the hIFN-α Standard are pipetted into these wells, followed by the addition of the Lucia Conjugated Antibody (detection antibody).
During the first incubation, the hIFN‑α antigen binds to the immobilized hIFN-α Capture Antibody on one site, and during the second incubation, to the Lucia Conjugated Antibody on a second site.

After removal of the excess detection antibody, QUANTI-Luc™, a solution that contains the substrate of Lucia luciferase, is added. No incubation is required.
The bioluminescent signal is emitted instantaneously and measured using a luminometer. The intensity of this signal is directly proportional to the concentration of hIFN-α present in the samples.

 

 

 

 

 

 

 

 

 

 

LumiKine™ kits
Type I Interferons
LumiKine™ hIFN-α	Human IFN-alpha (multiple subtypes) bioluminescent ELISA kit	5 plates
LumiKine™ mIFN-α	Mouse IFN-alpha (multiple subtypes) bioluminescent ELISA kit	5 plates
LumiKine™ hIFN-β	Human IFN-beta bioluminescent ELISA kit	5 plates
LumiKine™ mIFN-β	Mouse IFN-beta bioluminescent ELISA kit	5 plates
Interleukin-1
LumiKine™ mIL-1α	Mouse interleukin 1 alpha bioluminescent ELISA	5 plates
LumiKine™ hIL-1β	Human IL1-beta bioluminescent ELISA kit	5 plates
LumiKine™ Xpress kits
Type I Interferons
LumiKine™ Xpress hIFN-α	Human IFN-alpha (multiple subtypes) bioluminescent ELISA kit	5 plates
LumiKine™ Xpress mIFN-α	Mouse IFN-alpha (multiple subtypes) bioluminescent ELISA kit	5 plates
LumiKine™ Xpress hIFN-β	Human IFN-beta bioluminescent ELISA kit	5 plates
LumiKine™ Xpress mIFN-β	Mouse IFN-beta bioluminescent ELISA kit	5 plates
Interleukin-1
LumiKine™ Xpress hIL-1α	Human IL-1alpha bioluminescent ELISA kit	5 plates
LumiKine™ Xpress mIL-1β	Mouse IL-1beta bioluminescent ELISA kit	5 plates

 

 

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