LifeSensors社 Anti-K48 TUBEs
K48結合型ポリユビキチン鎖を特異的に検出
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitylated proteins, allowing detection at relatively low abundance. TUBEs effectively "capture" proteins in their polyubiquitylated state [1-3].
The affinity of Anti-K48 TUBE for K48 linked tetra-ubiquitin is approximately 50-fold higher than its affinity for K63- linked tetra-ubiquitin. His6 Anti-K48 TUBE can be used to isolate, enrich, and identify ubiquitylated proteins from cells, tissues, and organs.
Application:
- Identification of the polyubiquitin linkage-type of your protein of interest
- Inhibition of K48-dependent processes in lysates
- Purification of K48-linked polyubiquitinylated proteins from cell and tissue lysates
- In situ labeling for detection of K48-linked polyubiquitinylated proteins by histochemistry
- Protection of poly-ubiquitylated proteins from both deubiquitylation and degradation by the proteasome
Benefit
- Selective for K48-linked polyubiquitin chains
- Superior to a standard antibody-based methods
- High sensitivity
- Low cost per experiment
Product Information
Affinity tag: His6-SUMO
Purity:> 90% by SDS-PAGE
Molecular Weight: 37,825 Da
Physical State: liquid
Quantity: 75μg
Solubility: >30 mg/mL
Storage: -80℃. Avoid repeated freeze/thaw cycles
Detection of Tetra-Ubiquitin Chains via Far Western Blotting. (Left) Increasing amounts (10-
100ng) of Tetra K48-linked ubiquitin. (Right) To demonstrate the specificity of biotin anti-K48 TUBE we ran 50ng of K63, Linear and K48-linked Ub4. The blot was probed with 10μg/mL biotin anti-K48 TUBE in TBHS-T containing 1% BSA. After washing, the blot was incubated with avidin-HRP at 1:5000.
| Product Name | Catalog# | Size |
| UM205 | 75µg | |
| Anti-K48 TUBE, Biotin | UM305 | 200µg |
| Anti-K48 TUBE, Agarose | UM405 | 1ml |
| Anti-K48 TUBE, Flag | UM605 | 75µg |
製品概要
Fount of Information は、新商品、新規取扱メーカーなどの情報をいち早く紹介するコンテンツです。情報発信のスピードを重視しているコンテンツのため、現時点で法規制や取り扱いを確認できていない商品、定価を設定できていない商品があります。ご要望やご照会を受けた商品について、法令整備や在庫の充実を図ります。
