この製品に関するご意見・ご照会・お問合せはこちら

Over-Expression Cell Lysate: An Ideal Control

Getting a well characterized positive control is challenging especially when you are working on a unique protein that is not commonly expressed. 

OriGene's over-expression cells lysate collection includes 20,000 cell lysates of full length human proteins expressed in HEK293T cells.

Key features

• C-Terminal myc-DDK tag
  • Dual detection: Can be detected by both anti-myc-DDK and target antibody
  • An efficient handle for protein purification
• Expressed in HEK293T: Protein behavior very close to natural in-vivo conditions
• Characterized: Protein expression confirmed by Western Blot.  Watch this video!

Application

• Antibody Validation 
• Protein functional Assay
• Immuno-precipitation, protein-protein interaction

List of popular over-expression cell lysates

• Ovarian Cancer
• COVID-19 affected genes
• Whole cell lysates of cancer lines (NCI-60 collection)

Why expression hosts selection is critical for recombinant  protein synthesis?

One of the challenges of studying  a protein is to get a large quantities that close to the native protein.  Proteins expressed in  human cells such as HEK293  demonstrate

• Preservation of protein structure, post translational modifications, & functions
• Ideal for antibody production
• Studying protein-protein interaction
      

Learn more from this short video on 'Expression Hosts for Recombinant Proteins'.

Also for you

• Explore the Cytokines for Cancer Immunotherapy
• Listen to this short podcast on Cancer Diagnostics and PD-L1.

Interested references

• COVID-19 Worldwide Dashboard
• Review:  The trinity of COVID-19:  immunity, inflammation and intervention