l The ultimate solution to the never-ending struggle against microbial airborne, waterborne, and
l All-round protection to your tissue culture plants against contaminants without impairing in vitro
seed germination, callus proliferation, and callus regeneration.
l Prevents the germination of both bacteria and fungi spores
l Reduce/eliminate endogenous contamination
How to Use PPM™
Follow the given steps to prepare the tissue culture media involving PPM™ and provide the protection
your little tissue culture plants need:
l Take a beaker and add 800 ml of sterile distilled water to it.
l To the water, add PCT’s MS Media 4.54 grams (you don’t need to measure it as it comes in a
measured bottle), 30 grams of sugar, and PPM™ (generally 1-2 ml/L of media based on the plants
and contamination level.
l Add water to make 950 ml of the volume of the media.
l Adjust the pH of the media between 5.8-6.0.
l Add PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
l Make up the volume of the media to 1000ml.
l Autoclave the medium for 20 minutes at 15 psi and 121℃.
Your media is ready!