l Resistant to repeated high-temperature autoclave cycles
l Virtually unbreakable in regular use
l Microporous vent filter, helping prevent contamination
l Space and time efficient
l Widely used in tissue culture application
l Can multiply plants in hundreds and thousands of number
l Successfully tested for a wide range of plants, including orchids, cannabis, aquatics, succulents,
ferns, and carnivorous plants.
How to Use The Biocoupler™
Follow the given steps best to utilize the Biocoupler™ in your tissue culture application:
l Prepare liquid tissue culture media using PCT’s MS media and add sugar based on the quantity of
media you’re making (generally, for 1-liter media, 30 grams of sugar is added). (DO NOT add Agar).
l Take the Biocoupler™ and connect two Mason jars at their two ends.
l Measure 200 ml of prepared MS media.
l Open one side of the Biocoupler and add the measured 200 ml media to it.
l As you have prepared 1000 ml media, so can prepare a set of 5 Biocoupler with 200 ml MS media
l After filling the jars, close the Biocouplers loosely and autoclave them!
Open the Biocoupler™ under the laminar hood and transfer your plants into it after autoclaving. That’s it!