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PRIMORIGEN BIOSCIENCES社 骨髄由来間葉系幹細胞

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Description:

Mesenchymal Stem Cells (MSC) are high quality primary cells isolated from the bone marrow or adipose tissue of adult rats/mice using standardized methods. MSC are useful for studying cellular differentiation and transdifferentiation mechanisms. Cultured cells will form a monolayer after approximately 7 days, and can be passaged 10-12 times.

 

Characterization of cells:

Due the lack of a single marker to clearly define MSC, each lot has been validated using several physical, phenotypic and functional properties. The classical assay utilized to identify MSCs is the colony forming unit (CFU) assay that identifies adherent spindle shaped cells that proliferate to form colonies and can be induced to differentiate into adipocytes, osteocytes, and chondrocytes (see refs. 1-3).

 

Quality control:

Each lot of mesenchymal stem cells is negative for mycoplasma and bacteria, and have been verified to meet quality specifications in accordance with testing guidelines.

 

Recommended media:

The recommended media for mesenchymal stem cells are MSC (Adipose) Medium or MSC (Bone Marrow) Medium. Visit www.primorigen.com/cellprotocols for protocols and www.primorigen.com/media for media formulations.

Warranty:

Primorigen Biosciences warrants that the product will meet or exceed specifications when used with approved media and supplements. We will replace the product if the product does not conform to specifications. Our obligation and your sole remedy are limited to such replacement in the event that the product proves to be defective. Primorigen Biosciences is not liable for consequential damages.

 

References:

1. Lennon DP, Caplan AI. 2006. Isolation of rat marrow-derived mesenchymal stem cells. Exp Hematol. 34(11):1606-7. 2. Prockop DJ. 1997. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276: 71-74. 3. Bianco P, Gehron Robey P. 2000. Marrow stromal stem cells. J Clin Invest 105: 1663-1668.

 

Product Name

Product No.

Size

Cryo-preserved cells (Mouse)

S2238

1 x 106

Cryo-preserved cells (Rat)

S2213

1 x 106

Fixed on coverslips (Mouse)

S2239

3 slides

Fixed on coverslips (Rat)

S2214

3 slides

Cell lysate (Mouse)

S2240

100μg

Cell lysate (Rat)

S2215

100μg

Total RNA (Mouse)

S2251

10μg

Total RNA (Rat)

S2260

10μg

 

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