Cytoskeleton社 Rho経路活性化剤と阻害剤
細胞接着、細胞運動、細胞質分裂など、細胞骨格制御研究に
The G-switch™ line of small G-protein tools has been developed with an emphasis on creating highly potent reagents that target endogenous Rho family proteins and pathways. In contrast to methods that rely on over-expression or knockdown of target proteins (e.g., DNA transfection of dominant-negative or constitutively-active Rho mutants, RNAi knockdown), G-switch™ reagents act rapidly on the endogenous target protein (in minutes to hours, depending on the product), thereby optimizing the chance of generating a more physiologically relevant response. The G-switch™ product line includes reagents that directly and indirectly modulate Rho family signal transduction, thereby offering a wide range of mechanistic tools to study these critical cellular functions. See Cytoskeleton's web site for the latest G-switch™ information.
Product Uses Include
- Rho pathway studies in tissue culture cells
- Study effects of altered Rho pathway on other connected pathways
- An active control when testing other substances
Specification of Activators:
|
Activator |
Advantage |
Disadvantages |
Window of activation |
Serum compatible |
|
G-switch™ Type: Direct
|
The most robust activator for Rho available Sustained activation of Rho GTPases for >2hr Eliminates Ras, PI3K and PLC concomitant activation Most suitable activator for studying specific signal transduction pathways downstream of the Rho GTPases |
Cannot be used to study GAPs C3 exoenzyme (Cat# CT04) will not inhibit CN03 activated small G-proteins |
2 - 12 h, no signal after 24 h*. |
YES |
|
G-switch™ Type: Direct, |
The most robust activator for Rac and Cdc42 available, second only to CN03 for Rho activation Sustained activation of Rho, Rac and Cdc42 for >2hr Eliminates Ras, PI3K and PLC concomitant activation Excellent activator for studying signal transduction pathways downstream of Rho, Rac and Cdc42 |
Cannot be used to study GAPs C3 exoenzyme (Cat# CT04) will not inhibit CN03 activated small G-proteins |
Rho; 2 - 12 h, no signal after 24 h*. Rac; 2 - 24 h, no signal after 48 h. Cdc42; 2 - 24 h, no signal after 48 h. |
YES |
|
Rac/Cdc42 Activator Type: Indirect, |
Physiological stimulus Useful in studying pathway between cell surface and small G-proteins |
Very transient activation Ras, PI3K and PLC also activated |
1 - 3 min, no signal after 5 min. |
YES |
|
Rho Activator I Type: Indirect |
More sustained Rho activation than EGF (Cat# CN02) Eliminates Ras, PI3K and PLC concomitant activation |
Also inhibits calpain Inhibition of SHP-2 may have other biological consequences Requires DMSO vehicle |
10 - 30 min, no signal after 120 min. |
YES |
|
* = 24 h and longer Rho activation varies by cell type. |
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Product information
|
Product |
Product No. |
Description |
|
Rho Activator II |
Cell permeable and specific for Rho, recommended for use in Rho pathway studies or as a cell activator control. |
|
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Rho Inhibitor I |
Cell permeable and specific for Rho, recommended for use in Rho pathway studies or as a cell inhibitor control. |
|
|
Rho/Rac/Cdc42 Activator I |
Cell permeable and specific for Rho family, recommended for use in Rho family pathway studies or as a cell activator control. |
|
|
Rho Activator I |
Inhibits SHP-2 which indirectly activates Rho; also activates several other pathways (more info), only recommended as a cell activator control (not for pathway studies). |
|
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Rac/Cdc42 Activator II |
Binds EGF receptor and activates several pathways, including indirect activation of Rho; only recommended as a cell activator control (not for pathway studies). |
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C3 Transferase Protein |
An ADP-ribosyl transferase that selectively ribosylates Rho proteins in the effector-binding domain on asparagine 41, rendering them inactive. |
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