COSMOSIL HPLC Columns

COSMOSIL HILIC

COSMOSIL HILIC is a new column for hydrophilic interaction chromatography with Triazole bonded silica packing material. The hydrophilic interaction chromatography is a variation of normal phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of water miscible organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called “aqueous normal phase”, the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity. Without using ion-pair reagent COSMOSIL HILIC retains highly polar analytes that would not be retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with the positively charged stationary phase, thus acidic compound is strongly retained.

Material characteristics

Packing material HILIC
Silica gel high purity porous spherical silica
Average particle size 5 m
Average pore size approx. 120
Specific surface area approx. 300 m2/g
Stationary phase HILIC
Interactions hydrophilic interaction, anion exchange
Object substance hydrophilic compound, acidic compound
Feature Suitable for non-retaining by C18

Comparison with C18

COSMOSIL HILIC can separate glycine and glycylglycine without ion-pair reagent. Although C18 column can separate them with ion-pair reagents, there are some disadvantages such as column equilibration, preparation of mobile phase and column deterioration.

COSMOSIL HILIC COSMOSIL 5C18-PAQ
COSMOSIL HILIC COSMOSIL 5C18-PAQ

Different interactions

The retention Mechanism of COSMOSIL HILIC is the combination of hydrophilic interaction and anion exchange, and the retention can be controlled by changing the mobile phase.

Separation by hydrohilic interaction
Separation by hydrohilic interaction

Separation by anion exchange ability

Separation by anion exchange ability
Separation by anion exchange ability
BCAA(amino acid branched*chain)
BCAA(amino acid branched*chain)
Juice components
Juice components
Phosphorylated saccharide
phosphorylated_saccharide
Water-soluble vitamin
water_soluble_vitamin
Hydrophilic drug
Hydrophilic drug
Natural product
Natural product
GABA(4-Amino-n-butyric Acid)
GABA(4-Amino-n-butyric Acid)
Cyanoacetic Acid
Cyanoacetic Acid

Melamine analysis

Melamine analysis by HPLC and LC/MS/MS using COSMOSIL HILIC.

Melamine analysis

Combination with C18 columns

 
Combination with C18 columns

Optimizing analytical conditions

COMSOSIL HILIC Chromatogram Index, which includes 154 chromatograms using COSMOSIL HILIC. This index is useful for optimizing analytical conditions for hydrophilic interaction chromatography.

Optimizing analytical conditions

For more information on COSMOSIL 3.0 mm I.D. columns

Ordering information

Analytical and preparative column (Particle size : 5 μm)
COSMOSIL HILIC
Product name Size Product No.
COSMOSIL HILIC Guard Column
4.6 mm l.D.×10 mm 07055-61
10 mm l.D.×20 mm 07058-31
20 mm l.D.×20 mm 07854-91
20 mm l.D.×50 mm 07873-41
28 mm l.D.×50 mm 07874-31
COSMOSIL HILIC Packed Column
1.0 mm l.D.×150 mm 07869-11
1.0 mm l.D.×250 mm 07870-71
2.0 mm l.D.×50 mm 07052-91
2.0 mm l.D.×100 mm 08569-11
2.0 mm l.D.×150 mm 07054-71
2.0 mm l.D.×250 mm 07489-91
3.0 mm l.D.×150 mm 07871-61
3.0 mm l.D.×250 mm 07872-51
4.6 mm l.D.×150 mm 07056-51
4.6 mm l.D.×250 mm 07057-41
10 mm l.D.×250 mm 07059-21
20 mm l.D.×250 mm 07060-81
28 mm l.D.×250 mm 07875-21

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