モノクローナルTag抗体
Anti-GFP(Rat IgG2a), Monoclonal(GF090R), CC
Tag 抗体を用いることで、遺伝子組み換え技術を用いて人為的に導入したTag 標識をもつタンパク質の検出や精製が容易となります。これらTag 抗体を用いることで目的タンパク質に対する抗体を作製するという手間を省くことができ、タンパク質の局在、タンパク質間相互作用、タンパク質機能解析など多くの研究目的において利用されています。
その他未標識のTag抗体はこちら
- Anti-GFP(Mouse IgG1-κ), Monoclonal(GF200), AS
- Anti-HA(Mouse IgG1-κ), Monoclonal(HA124), AS
- Anti-6xHis(Mouse IgG1-κ), Monoclonal(HI192), AS
- Anti-c-Myc(Mouse IgG1-κ), Monoclonal(MC045), AS
アガロース結合Tag抗体、POD標識Tag抗体はこちら
製品説明
製品説明
Clone | GF090R |
---|---|
Isotype | IgG2a(Rat) |
Product form | Liquid |
Immunogen | His-GFP(full length)fusion protein |
Application | Immunohistochemistry 1:1,000-1:2,000 Western Blotting 1:1,000-1:2,000 ELISA 1:2,000-1:20,000 |
顧客使用例
使用例 1
免疫組織染色
細胞膜移行シグナルを付加した EGFP を、Anti-GFP(Rat IgG2a)モノクローナル抗体で組織染色したものです。
サンプル | : | マウス脳(副嗅球の神経細胞) |
一次抗体 | : | Anti-GFP(Rat IgG2a), Monoclonal(1:1,000) 室温 over night |
二次抗体 | : | Anti-Rat IgG-Cy3(1:300) 室温 1 hr |
ブロッキング | : | 5% normal goat serum / 0.2% TritonX-100 in PBS |
固定法 | : | 4% パラホルムアルデヒド |
本データは理化学研究所 脳科学総合研究センター シナプス分子機構研究チーム チームリーダー 吉原 良浩 様よりご提供いただきました。
使用例 2
ウェスタンブロッティング
サンプル | : | ①GFP ②GFP-14-3-3 (293 cell lysate 50 μg) |
ゲル | : | 5-20% Gradient Gel |
一次抗体 | : | Anti-GFP(Rat IgG2a), Monoclonal(3 μg/mL) over night |
二次抗体 | : | Anti-Rat IgG-HRP(1:10,000) |
本データは東京医科歯科大学 難治疾患研究所 分子遺伝分野 准教授 吉田 清嗣 様よりご提供いただきました。
使用文献
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- Abe, K. et al. Loss of Heterochromatin Protein 1 Gamma Reduces the Number of Primordial Germ Cells via Impaired Cell Cycle Progression in Mice. Biol Reprod 85(5), 1013-24 (2011).
- Hide, T. et al. Combination of a ptgs2 inhibitor and an epidermal growth factor receptor-signaling inhibitor prevents tumorigenesis of oligodendrocyte lineage-derived glioma-initiating cells. Stem Cells. 29(4), 590-9 (2011).
- Kurita, S. et al. Cooperative Role of Nectin-Nectin and Nectin-Afadin Interactions in Formation of Nectin-based Cell-Cell Adhesion. J Biol Chem. 286(42), 36297-303 (2011).
- Wang, X. et al. Loss of adenomatous polyposis coli in Bergmann glia disrupts their unique architecture and leads to cell nonautonomous neurodegeneration of cerebellar Purkinje neurons. Glia. 59(6), 857-68 (2011).
- Mochizuki, N. Intravenous injection of neural progenitor cells improves cerebral ischemia-induced learning dysfunction. Biol Pharm Bull. 34(2), 260-5 (2011).
- Inamura, N. et al. Olig2 lineage cells generate GABAergic neurons in the prethalamic nuclei, including the zona incerta, ventral lateral geniculate nucleus and reticular thalamic nucleus. Dev Neurosci. 33(2), 118-29 (2011).
- Vidaki, M. et al. Rac1-Dependent Cell Cycle Exit of MGE Precursors and GABAergic Interneuron Migration to the Cortex. Cereb Cortex. (2011).
- Konno, A. et al. Evaluation of a Sindbis virus vector displaying an immunoglobulin-binding domain: Antibody-dependent infection of neurons in living mice. Neurosci Res. (2011).
- Gotoh, H. et al. Genetically-defined lineage tracing of Nkx2.2-expressing cells in chick spinal cord. Dev Biol. 349(2), 504-11 (2011).
- Rubin, AN. et al. The germinal zones of the basal ganglia but not the septum generate GABAergic interneurons for the cortex. J Neurosci. 30(36), 12050-62 (2010).
- Matsumoto, H. et al. Spatial arrangement of glomerular molecular-feature clusters in the odorant-receptor class domains of the mouse olfactory bulb. J Neurophysiol. 103(6), 3490-500 (2010).
- Tamura, H. et al. Atg9A Protein, an Autophagy-related Membrane Protein, Is Localized in the Neurons of Mouse Brains. J Histochem Cytochem. 58(5), 443-53 (2010).
- Asai, R. et al. Endothelin receptor type A expression defines a distinct cardiac subdomain within the heart field and is later implicated in chamber myocardium formation. Development. 137(22), 3823-33 (2010).
- Hirota, Y. et al. Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. Development. 137(18), 3037-46 (2010).
- Honma, Y. et al. Axonal projections of mechanoreceptive dorsal root ganglion neurons depend on Ret. Development. 137(14), 2319-28 (2010).
- Kohyama, J. BMP-induced REST regulates the establishment and maintenance of astrocytic identity. J Cell Biol. 189(1), 159-70 (2010).
- Manno, A. et al. Enhanced ATPase activities as a primary defect of mutant valosin-containing proteins that cause inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia. Genes Cells. 15(8), 911-22 (2010).
- Mains, RE. et al. Kalrn promoter usage and isoform expression respond to chronic cocaine exposure. BMC Neurosci. 12, 20 (2011).
関連資料
価格表
関連製品
その他未標識のTag抗体はこちら
- Anti-GFP(Mouse IgG1-κ), Monoclonal(GF200), AS
- Anti-HA(Mouse IgG1-κ), Monoclonal(HA124), AS
- Anti-6xHis(Mouse IgG1-κ), Monoclonal(HI192), AS
- Anti-c-Myc(Mouse IgG1-κ), Monoclonal(MC045), AS
アガロース結合Tag抗体、POD標識Tag抗体はこちら