COSMOGEL Ig-Accept Series
COSMOGEL® Ig-Accept Protein A / COSMOGEL® Ig-Accept Protein G
These products utilize Protein A or Protein G binding to the Fc region of immunoglobulins (Ig) for the purification of IgG.
Application 1
Purification of 35S-labeled SEL1L by Immunoprecipitation
Data courtesy of a researcher from Kyoto University
Description
Each product was evaluated by purification of 35S-labeled SEL1L overexpressed in HEK293 cells by Immunoprecipitation.
Procedure
- Rabbit anti-SEL1L polyclonal antibody was introduced to an extract of HEK293 cells which had 35S methionine and 35S cysteine pre-incorporated, and the mixture was then incubated.
- Protein A or Protein G, sourced from Company A, as well as COSMOGEL® Ig Accept, were respectively added to separate samples and incubated.
- After washing, laemmli buffer was added and treated at 65℃ .
- The supernatant was applied to a SDS-PAGE gel and the signal was detected by Typhoon.
Results / Evaluation
Lane:
- Protein A from company A
- COSMOGEL® Ig Accept Protein A
- Protein G from company A
- COSMOGEL® Ig Accept Protein G
The results of the experiment were analyzed by subtracting the background, as indicated by red squares 2, 4, 6, and 8, from the signal intensity of the target protein , corresponding to red squares 1, 3, 5, and 7, respectively.
User Evaluation
The signal intensity of the target protein for Ig Accept was comparable to that of Company A’s product. A nonspecific band ( ★ ) was detected on the low molecular weight side in the case of Protein G from Company A, but was absent in COSMOGEL® Ig Accept.
Application 2
COSMOGEL® Ig-Accept Protein A
Sample:
Lane 3-4: Human serum (ammonium sulfate precipitation fraction)
Lane 6-7: Mouse serum (ammonium sulfate precipitation fraction)
Elution: Glycine - Hydrochloric acid buffer (pH 2.8)
Line:
1. Protein Markers (10x) (#29458-24) in 1x
2. Human serum (10x dilution)
3. Sample purified using COSMOGEL® Ig-Accept Protein A
4. Sample purified using Protein A carrier from Company A
5. Mouse serum (10x dilution)
6. Sample purified using COSMOGEL® Ig-Accept Protein A
7. Sample purified using Protein A carrier from Company A
COSMOGEL® Ig-Accept Protein G
Sample:
Mouse serum (ammonium sulfate precipitation fraction)
Elution: Glycine - Hydrochloric acid buffer (pH 2.8)
Line:
1. Protein Markers (10x) (#29458-24) in 1x
2. Sample purified using COSMOGEL® Ig-Accept Protein G
Product Specifications
| Carrier Name | COSMOGEL® Ig-Accept Protein A |
COSMOGEL® Ig-Accept Protein G |
|---|---|---|
| Ligands | Protein A | Protein G |
| Particle size | 50 - 150 μm | |
| Matrix | 4% cross-linked agarose | |
| Recommended Line Velocity | 26 cm/h | |
| Limited pressure | 3.6 psi （0.25 bar） | |
| Form | 50 vol% solution (20 vol% ethanol solution) | |
Ordering Information
| Product Name | Grade | Storage | Product Number | PKG Size | Price |
|---|---|---|---|---|---|
| COSMOGEL® Ig-Accept Protein A | SP | R | 02179-14 | 5 mL |  |
| 02179-72 | 25 mL | ||||
| COSMOGEL® Ig-Accept Protein G | SP | R | 02198-44 | 5 mL | |
| 02198-22 | 25 mL |
