Products

Silica Gel for Column Chromatography

Silica Gel

Since conventional silica gels are weakly acidic, some pH sensitive compounds may be decomposed during the purification by column chromatography with the acidic silica gels. The pH of Silica Gel 60 (spherical neutral) is adjusted to nearly neutral for the separation of not only pH sensitive compounds but also new compounds that the physical properties are still unknown.
*All chromatograms shown below are obtained with silica gel packed into stainless and steel columns.

Comparison with conventional silica gel

Purification of Acetal

Influence of the particle size on peak shape

Condition
Column size 4.6 mm I.D. x 250 mm Sample Sample A (100 mg/ml)
SampleA
Mobile phase ethyl acetate : hexane = 1 : 99
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm, 0.08AUFS
Internal standard Methyl Benzoate (10mg/ml) Injection vol. 3 µl

Influence of the particle size on peak shape

Condition
Column size 4.6 mm I.D. x 250 mm Sample Sample B (200 mg/ml)
SampleA
Mobile phase ethyl acetate : hexane = 1 : 99
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm, 0.08AUFS
Internal standard Methyl Benzoate (10 mg/ml) Injection vol. 3 µl

Ordering information

Silica Gel 60 (spherical, neutral)

Product Name PKG size Product No.
Silica Gel 60, spherical, neutral, avg. particle size 75 µm
100 g 30511-64
500 g 30511-35
1 kg 30511-51
5 kg 30511-06
25 kg 30511-22
Silica Gel 60, spherical, neutral, avg. particle size 140 µm
100 g 30518-94
500 g 30518-65
1 kg 30518-81
25 kg 30518-52

Silica gel (for column chromatography)

Ordering information

Silica gel (irregular)

Product Name Particle size PKG size Product No.
Silica gel 60
approx. 70-230 mesh 500 g 30724-55
1 kg 30724-71
5 kg 30724-84
25 kg 30724-42
approx. 230-400 mesh 500 g 30721-85
1 kg 30721-01
5 kg 30721-14
25 kg 30721-72

Silica gel (spherical)

Product Name Particle size PKG size Product No.
Silica gel 60, spherical
approx. 70-230 mesh 1 kg 30731-71
25 kg 30731-42
approx. 150-325 mesh 1 kg 30733-51
25 kg 30733-22
Silica gel 120, spherical approx. 70-230 mesh 1 kg 30734-41
approx. 150-325 mesh 1 kg 30735-31

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze