Protein Ladder One Plus, Triple-color for SDS-PAGE
The product is a pre-stained ladder marker using recombinant proteins. It consists of 14 bands, enabling detailed analysis of molecular weight.
- Excellent visibility with triple color
- Sharp bands
- Consists of bands at many different MW
- Low lot-to-lot variation
Compared to other brands with same molecular weight range, this product shows more bands and higher visibility for different molecular weights.
|Product Name||Protein Ladder
|Company A||Company B|
|Gel||Extra PAGE One Precast Gel, 5-20%, 13wells (#13063-74)
After electrophoresis Load volume
(recommended amount for mini-gel)
|5 μL||10 μL||5 μL|
|Number of bands||14||10||12|
|Volume (product package)||500 μL||500 μL||500 μL|
|Number of uses (mini-gel)||100 lanes||50 lanes||100 lanes|
Application 1: Comparison based on electrophoresis gel
The product shows sharp bands from low to high molecular weight on different gels.
|Gel||Handmade mini-gel (Laemmli method)||Extra PAGE One Precast Gel||Bullet PAGE One Precast Gel|
|Gel concentration||12%||5 - 20%||5 - 15%|
|Load volume||5 μL|
Application 2: Transfer in Western Blotting
The result can be seen clearly on the PVDF membrane. Furthermore, the product also can be used with fluorescence detection.
Under red and far-red excitation, blue and green bands can be detected. *Red band (75 kDa) does not show under fluorescence detection. When using this product under fluorescence detection, please dilute to about 1/10 of the regular concentration.
|SDS-PAGE||(Gel) Extra PAGE One Precast Gel, 5-20%, 13wells (#13063-74)
(Sample) Protein Ladder One Plus 1; 5 μL 2; 2.5 μL
Protein Ladder One Plus (diluted 10x*) 3; 5μL
* Use sample buffer (1x) to dilute (without heating)
|Transfer||(Membrane) 1, 2; PVDF membrane 3 Low-fluorescence PVDF membrane
(Transfer buffer) Semi-dry Blotting Buffer Solution for Western Blotting (#30650-31)
(Transfer condition) 10 V (Constant voltage) 45 mins
|ChemiDoc Touch MP (Bio-Rad)
Red excitation: 700 nm (width 50 nm) filter; Exposure time: 5 seconds
Far-red excitation: 715 nm (width 30 nm) filter; Exposure time: 30 seconds
- There is a possibility of different molecular weight result depending on gel type (concentration, gradient, buffer, etc.). It may not suitable for accurate molecular weight measurement.
- The product is ready-to-use type. Sample preparation, such as heating and reduction, is not required.