Products

Rapid Running Buffer Solution(20x) for SDS-PAGE

A running buffer for hand-made SDS-PAGE gels, which is able to dramatically shorten protein migration time. Please note that the product is designed for hand-made gels. Any commercially available precast gels should be evaluated beforehand.

Features

    • Time-saving: Approximately 25-minute protein migration time with mini-gel on 250 V
    • Simple: Replace Laemmli running buffer with Rapid Running Buffer Solution
    • Gradient gel-like separation pattern: Wider separation range
    • High transfer efficiency: Lower acrylamide concentration gel recommended
    • Applicable to Wide Range Gel*

      *Wide Range Gel: Hand-made gels casted with Wide Range Gel Preparation Buffer, Catalog No. 07831-94, which offer gradient gel-like separation pattern -- the gradient in pore size offers wide range of molecular weights separation in a single run -- and also have tensile strength character.

Product Information

This running buffer offers shorter protein migration time than Laemmli running buffer. No significant difference of buffer temperature increase with Laemmli running buffer shows.

Comparison of protein migration time and buffer temperature with Laemmli running buffer

eidoujikan.jpg

Gel consentrationRunning buffer
8% Rapid Running Buffer
12% Laemmli Running Buffer (0.025 mol/l tris,
0.192 mo/l L-glysin and 0.1% SDS)

Comparison of separation pattern on the same acrylamide percentage gel

Gel concentration 8% 8%
Running buffer Rapid Running Buffer Laemmli running buffer
Transfer time required 23 minutes

Separation pattern

Tips

Lower acrylamide concentration required

To have a similar separation pattern running with Rapid Running Buffer, 4% lower acrylamide concentration should be used as the below table.

Laemmli method
Gel concentration Running buffer
12% Laemmli running buffer

yajirusi2.jpg

Saving-time protocol
Gel concentration Running buffer
8% Rapid Running Buffer

Separation pattern

Gel
  • Laemmli mini-gel
  • Wide Range mini-gel
Running buffer
  • Rapid Running buffer
  • Laemmli running buffer
Sample Protein ladder manufactured by Nacalai, #29458-24
Voltage 250 V
Gel staining CBB Stain One(#04543)

Laemmli gel run with Rapid Running Buffer

Running buffer Rapid Running Buffer
Gel Laemmli gel
Gel concentration 6% 8% 10% 4-10%
Separation pattern honseihin_laemmli_6.jpg honseihin_laemmli_8.jpg honseihin_laemmli_10.jpg honseihin_laemmli_4-10.jpg

Laemmli gel run with Laemmli running buffer

Running buffer Laemmli running buffer
Gel Laemmli gel
Gel concentration 6% 8% 10% 12% 15%
Separation pattern laemmli_laemmli_6.jpg laemmli_laemmli_8.jpg laemmli_laemmli_10.jpg laemmli_laemmli_12.jpg laemmli_laemmli_15.jpg

Wide Range Gel run with Rapid Running Buffer

To separate high molecular weight area, Wide Range Gel will be helpful.

Running buffer Rapid Running Buffer
Gel WIDE RANGE Gel
Gel concentration 4% 6% 8% 10%
Separation pattern honseihin_WIDE_4.jpg honseihin_WIDE_6.jpg honseihin_WIDE_8.jpg honseihin_WIDE_10.jpg

Application

2-D electrophoresis

Rapid Running Buffer is also applicable to 2-D electrophoresis.

nijiegn.jpg

Sample HL-60 cell extract
1-D Immobiline pH 4-7
2-D 10% Laemmli mini gel
Running buffer Rapid Running Buffer
Voltage 250 V、25minutes
Gel staining Sil-best Stain One manufactured by Nacalai, #06865-81

Immubiline® is a trademark by General Electric Company.

Transfer efficiency

Because of lower acrylamide concentration, protein transfer efficiency from a gel to a membrane will improve.

Gel concentration 8% 12% 8% 12%
Running buffer Rapid Running Buffer Laemmli running buffer Rapid Running Buffer Laemmli running buffer
Transfer time required 10 minutes 30 minutes

Membrane staining image

WB_honhin_10min.jpg

WB_laemmli_10min.jpg

WB_honhin_30min.jpg

WB_laemmli_30min.jpg

Gel Laemmli mini gel
Sample 1 lane Protein Ladder One, Triple-color, #09547-74, 5 μl
2-4 lane Protein Marker, #29458-24, 3x, 1x, 1/3x 2μl each
Voltage 250 V
Transfer buffer/Voltage Semi-dry Transfer Buffer, #30650-31/10 V
Menbrane staining CBB Stain One(#04543)

Note

  • Rapid Running Buffer is designed for hand-made gels, but not for Native-PAGE gels.
    Any commercially available precast gels should be evaluated beforehand.
  • Pre-stained marker bands might appear slightly bigger molecular weight than they actual have due to dye conjugation.
  • Actual protein migration time and buffer temperature increased may depend on a gel composition and its size, amount of running buffer, type of chamber, etc.

Video

Rapid Running Buffer Solution(20x) for SDS-PAGE

Download

Rapid Running Buffer Solution(20x) for SDS-PAGE pdf(PDF 2.4 MB)

Ordering information

Product NameGradeStorageCatalog NumberPKG Sizee-Nacalai

Rapid Running Buffer Solution

(20x) for SDS-PAGE

SP

Room

temperature

12981-74 250 mL e-Nacalai.jpg

Related products

Product NameCatalog NumberPKG Size
■ Gel casting reagents
40(w/v)%-Acrylamide/Bis Mixed Solution(29:1) 06119-45 500 ml
40(w/v)%-Acrylamide/Bis Mixed Solution(37.5:1) 06121-95 500 ml
30(w/v)%-Acrylamide/Bis Mixed Solution(29:1) 06141-35 500 ml
30(w/v)%-Acrylamide/Bis Mixed Solution(37.5:1) 06144-05 500 ml
10%-SDS Solution 30562-04 100 ml
N,N,N',N'-Tetramethylethylenediamine 33401-72 25 g
10(w/v)%-Ammonium Peroxodisulfate Solution 02634-34 10 ml
WIDE RANGE Gel Preparation Buffer(4x) for PAGE 07831-94 250 ml
■ Running buffer
Running Buffer Solution(10x) for SDS-PAGE, Tris-Glycine 30329-61 1 L
■ Sample preparation reagents
Sample Buffer Solution with 2-ME(2x) for SDS-PAGE 30567-12 25 ml
Sample Buffer Solution without 2-ME(2x) for SDS-PAGE 30567-12 25 ml
Sample Buffer Solution with Reducing Reagent(6x) for SDS-PAGE 09499-14 5 ml
Sample Buffer Solution without Reducing Reagent(6x) for SDS-PAGE 09500-64 5 ml
■ Gel staining products
Dispotray-S Outer size:150 mm x 105 mm x 19 mm Bottom face size: 122 mm x 80 mm 16526-82 25 pieces
Dispotray-M Outer size:200 mm×140 mm×25 mm Bottom face size: 155 mm×105 mm 16551-84 25 pieces
Rapid Stain CBB Kit 30035-14 1 set
CBB Stain One(Ready To Use) 04543-51 1 L
Sil-Best Stain One 06865-81 1 set