Serum-free culture mediumReduced Serum DMEM/Ham's F-12 Medium

In comparison to DMEM / Ham's F-12 medium, this product allows for cell cultivation with reduced serum supplementation, without affecting cell proliferation rate or morphology.


  • Cultivable under low-serum conditions
  • Suitable as a basal medium for serum-free cultivation
  • Reduce costs associated with serum usage
  • Minimize variations between batches, reducing serum-related effects


Comparison of cell proliferation rate and cell morphology

HEK 293 and CHO cells were cultured using both this product and Company A's serum-reduced medium. Similar to the medium from Company A, our product demonstrated that cultivation under low-serum conditions is feasible without affecting either cell proliferation rate or morphology。

Proliferation curves and cell morphology of HEK 293 cells cultured under various serum concentrations (Day 35)

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Proliferation curves and cell morphology of CHO cells cultured under various serum concentrations (Day 35)

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Cultivation of Colorectal Cancer Patient-Derived Organoids (Under Serum-Free Conditions)

We prepared droplets (n=4) embedding dispersed colon cancer patient-derived organoid cells using Corning® Matrigel Basement Membrane Matrix Growth Factor Reduced and TrypLE Express. Then, 1 mL of medium* was added on top, and the cultures were incubated.
*2 mM L-Alanyl-L-Glutamine was added to both of this product and Company A’s product.

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Visual assessment of proliferation did not reveal significant differences between the two conditions

Data courtesy of Associate Professor Junpei Kondo, Department of Molecular Biochemistry, Division of Health Sciences, Graduate School of Medicine, Osaka University

Ordering Information

Product Name Grade Storage Catalog Number PKG Size Price
Reduced Serum DMEM/Ham's F-12 Medium SP Refrigerate 21906-55 500 mL e-Nacalai