- Fast: Short blocking time
- Wide range: Contains both high molecular weight compounds and bovine serum proteins
- Easy to use: Simple storage in refrigerator even after the bottle is opened
Blocking is indispensable in immunoassays in order to block non-specific binding reactions. Blocking One contains both high molecular weight compounds and bovine serum protein. Blocking One is superior to conventional blocking solutions. The preservative in Blocking One does not affect the enzyme activity of peroxidase (POD) or alkaline phosphatase (ALP). Only simple refrigerator storage is necessary, even after opening the bottle.
Comparison of blocking reaction times
The relationship between reaction time and the effect of blocking in microplate assay. In comparison with other products, Blocking One shows by far the fastest dinamics.
Since the antigen reaction is omitted in this experiment, when staining occurs, it is caused by non-specific adsorption. The figure shows negative data
Linearity in quantitative protein analysis
This product does not contain preservative which affects the enzyme activity of Peroxidase (POD) or Alkaline Phosphatase (ALP). Using common enzyme (POD, ALP) gives good result with high linearity.
Detection of Mouse IgG(H+L) by Western Blotting
Incubation time for blocking : 20 min.
Sample M: Biotinylated Protein Marker (100 dilution, 5 μl)
1 : Mouse Serum (2.5 μl)
2 : Mouse Serum (5.0 μl)
3 : Mouse Serum (10 μl)
Mouse IgG (H+L) in Mouse Serum detected with Biotinylated anti-Mouse
Very little background occurs, indicating exceptional blocking performance.
Prevention of non-specific antibody binding in immunohistochemical application
Histological staining of normal organ tissue slices had been performed based on the following protocol, and the efficiency of different blocking agents wrere compared (20 minutes blocking time). The dots stained brown show Fibronectin existence. The key for successful observation is the contrast between the stained dots and the background, Blocking One gives the lowest background.
Blocking One Series (PDF 100 KB)
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Blocking One Series (PDF 1.2 MB)
|Product Name||Grade||Storage||Product No.||PKG Size||Price|
|Blocking One||SP||R||03953-95||500 ml|
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze