Products

Sialic Acid Related Products and Others

Substrates

  • N-Acetyl-D-mannosamine, Monohydrate [ManNAc]
  • N-Acetylneuraminic Acid [NANA; Sialic Acid; NeuAc]
  • Cytidine-5'-monophospho-N-acetylneuraminic Acid, Disodium Salt [CMP-Neu5Ac・2Na]
  • Uridine-5'-diphosphoglucose, Disodium Salt
  • Uridine-5'-diphosphoglucuronic Acid, Trisodium Salt

Enzymes

  • Neuraminidase (Sialidase)

Others

  • Sialic Acid derivative
  • Glucose derivative
  • Galactose derivative
  • Lactose derivative
  • Protected sialo-containing oligosaccharides
  • Hyaluronic acid

Substrates

N-Acetyl-D-mannosamine, Monohydrate [ManNAc]

N-Acetyl-D-mannosamine Monohydrate [ManNAc]

Origin Prepared by enzymatic hydrolysis of N-Acetylneuraminic Acid, and purified by crystallization.
Formula C8H15NO6 , H2O (MW:239.2)
Appearance White amorphous powder
Purity More than 99% by HPLC. Homogeneous by thin layer chromatographic analysis
Analytical Methods
  1. Thin layer chromatography (TLC)
    TLC plate Silica Gel 60 (Merck)
    Solvent system n-Propanol:Acetic acid:H2O (60:10:25)
    Color reagent Diphenylamine-aniline1)
  2. High performance liquid chromatography (HPLC) 2)
    Column Bio-Rad Aminex HPX-87H (4.6 mm ID x 300 mm) x2
    Temperature 65ºC
    Eluent 6 mMH2SO4
    Detection 205 nm
Storage Stable when stored on desiccated condition at 5ºC.
References
  1. K. Hotta, M. Kurokawa and S. Isaka, Seikagaku (in Japanese), 45 (10), 911-915 (1973)
  2. S. Blayer, J. M. Woodley, M. J. Dawson and M. D. Lilly, Biotechnology and Bioengineering, 66 (2), 131-136 (1999)

Ordering information

Product Name Grade Storage Product No. PKG Size
N-Acetyl-D-mannosamine Monohydrate SP R 05425-71 1 g
05425-84 10 g

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

N-Acetylneuraminic Acid [NANA; Sialic Acid; NeuAc]

N-Acetylneuraminic Acid [NANA; Sialic Acid; NeuAc]

Origin Prepared by enzymatic hydrolysis of colominic acid1), or enzymatic synthesis from N-acetylglucosamine and pyruvic acid.2)
Purified by ion-exchange column chromatography and crystallization.
Formula C11H19NO9 (MW : 309.27)
Appearance White crystalline powder
Purity More than 99% by colorimetric determination and HPLC
Analytical Methods
  1. Colorimetric assay
    TBA method3)4)is generally used to measure free sialic acid.
    Resorcinol method5)can be used to measure free, bound, or total sialic acid.>
  2. High performance liquid chromatography (HPLC)
    Column Bio-Rad Aminex HPX-87H
    Eluent 10 mM H2SO4
    Detection 205 nm
  3. Enzymatic assay
    NANA aldolase method6)
Uses Authentic specimen of the highest purity, substrate for NANA aldolase and starting material for preparing NANA derivatives.
Storage Stable for one year when stored on desiccated condition at 5 °C
References
  1. Y. Uchida, Y. T sukada, and T. Sugimori, Biochim. Biophys. Acta., 350, 425 (1974)
  2. I. Maru, J. Ohnishi, Y. Ohta and Y. Tsukada, Carbohydr. Res., 306, 575 (1998)
  3. Y. Uchida, Y. Tsukada and T. Sugimori, J. Biochem., 82, 1425 (1977)
  4. L. Warren, J. Biol. Chem., 234, 1971 (1959)
  5. J. T. Cassidy, G. W. Jourdian and S. Roseman, J. Biol. Chem., 240, 3501 (1965)
  6. H. Araki and M. Yamada, Methods of Enzymatic Analysis, VI, 80 (1984)

Ordering information

Product Name Grade Storage Product No. PKG Size
N-Acetylneuraminic Acid [NANA, Sialic Acid] GR F 08371-36 10 g
08371-94 100 g

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Cytidine-5'-monophospho-N-acetylneuraminic Acid, Disodium Salt [CMP-Neu5Ac・2Na]

Cytidine-5'-monophospho-N-acetylneuraminic Acid, Disodium Salt [CMP-Neu5Ac・2Na]

Origin Prepared by enzymatic synthesis from N-acetylneuraminic acid and CTP.
Purified by ion-exchange column chromatography and lyophilization.
Formula C20H29N4O16PNa2 (MW : 658)
Appearance White lyophilized powder
Purity More than 97% by HPLC
Analytical Methods

High performance liquid chromatography (HPLC)

Column Cosmosil 5C18-AR (6.0 mm ID x 150 mm)
Eluent 50 mM KH2PO4
Detection 254 nm
Storage Stable when stored on desiccated condition below -20°C

Ordering information

Product Name Grade Storage Product No. PKG Size
Cytidine-5'-monophospho-N-acetylneuraminic Acid Disodium Salt SP F 10432-24 10 mg

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Uridine-5'-diphosphoglucose, Disodium Salt

Uridine-5'-diphosphoglucose, Disodium Salt

Appearance White lyophilized powder
Purity More than 98% (Moisture : approx. 7%)
Analytical Methods
  1. Paper electrophoresis
    Buffer 0.1 M Ammonium acetate buffer, pH 5.2
    Condition 90 min at 2.5 mA / cm
    Detection UV lamp
  2. High performance liquid chromatography (HPLC)
    Column Shim-pack WAX-1 (4.0 mm ID x 50 mm)
    Eluent 20 mM Phosphate buffer (pH 7.0) / 480 mM phosphate buffer (pH 6.85) gradient elution
    Detection 260nm
  3. Determination
    The amount is calculated based on the molar extinction coefficient, ε262 = 10,000, measuring the optical density at 262 nm in 0.01 N HCl solution (pH 2.0)
Storage Stable when stored on desiccated condition at 5°C

Ordering information

Product Name Grade Storage Product No. PKG Size
Uridine-5'-diphosphoglucose Disodium Salt GR R 36001-64 100 mg
36001-51 1 g

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Uridine-5'-diphosphoglucuronic Acid, Trisodium Salt

Uridine-5'-diphosphoglucuronic Acid, Trisodium Salt

Formula C15H19N2O18P2Na3 (MW : 646.3)
Appearance White lyophilized powder
Purity More than 98% (Moisture : approx. 12%)
Analytical Methods
  1. Paper electrophoresis
    Buffer 0.1 M Ammonium acetate buffer, pH 5.2
    Condition 90 min at 2.5 mA / cm
    Detection UV lamp
  2. High performance liquid chromatography (HPLC)
    Column Shim-pack WAX-1 (4.0 mm IDx50 mm)
    Eluent 20 mM Phosphate buffer (pH 7.0)/ 480 mM phosphate buffer (pH 6.85) gradient elution
    Detection 260nm
  3. Determination
    The amount is calculated based on the molar extinction coefficient, ε262 = 10,000, measuring the optical density at 262 nm in 0.01 N HCl solution (pH 2.0)
Storage Stable when stored on desiccated condition at 5°C

Ordering information

Product Name Grade Storage Product No. PKG Size
Uridine-5'-diphosphoglucuronic Acid Trisodium Salt GR R 36002-54 100 mg
36002-41 1 g

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Enzymes

Neuraminidase (Sialidase)

Origin Arthrobacter ureafaciens
Reaction Sialyl compound→Sialic acid + Asialocompound
Appearance White amorphous powder
Activity More than 80 units/mg protein for N-acetylneuraminyllactose (NANA-lactose)
Unit definition One unit is the amount of enzyme required to liberate 1 µmol of N-acetylneuraminic acid (NANA) per minute at pH5.0 at 37 °C.
Storage Stable for one year when stored below 5°C.
For prolonged storage, keep at -20 °C.
Contaminations Enzyme activities mentioned below cannot be detected. 1)
Protease , N-Acetylneuraminic acid aldolase,
Glycosidase such as
α-glucosidase, β-glucosidase, α-galactosidase,
β-galactosidase, α-mannosidase, α-fucosidase,
N-Acetyl-α-glucosaminidase, N-Acetyl-β-glucosaminidase,
N-Acetyl-α-galactosaminidase, N-Acetyl-β-galactosaminidase,
N-Acetyl-α-mannosaminidase, N-Acetyl-β-mannosaminidase.
Properties 2) 3)
Molecular weight Approx. 52,000 Da, 66,000 Da and 88,000
(gel filtration, SDS-PAGE)
Optimum pH 4.5-5.5 (NANA-lactose as a substrate)
pH stability 4.5-9.5
Thermal stability below 60 °C (pH5.0, 20 min)
Substrate specificity The α-ketosidic linkage of N-glycolylneuraminic acid (NGNA) can be hydrolyzed as well as that of NANA. This enzyme cleaves α(2→3), α(2→6) and α(2→8) linkages of N-acetylneuraminic acid in glycoconjugates. The acivity is independent on Ca2+ and is not inhibited by EDTA, which is in striking contrast to Vibrio cholerae neuraminidase, and is not or slightly inhibited by inhibitors such as monoiodoacetate, p-chloromercuribenzoate and HgCl2, which is in striking contrast to Clostridium perfringens neuraminidase.
Assay Method Standard assay system is composed of 50 µl of substrate solution (4 mg/ml, NANA-lactose), 50 µl of 200 mM acetate buffer (pH5.0) and 100 µl of enzyme solution. Reaction is carried out at 37 oC for 10 minutes and NANA liberated is determined by thiobarbituric acid (TBA) method.1)-4)
References
  1. Y. Uchida, Y. Tsukada and T. Sugimori, J. Biochem., 82, 1425 (1977)
  2. Y. Uchida, Y. Tsukada and T. Sugimori, J. Biochem., 86, 1573 (1979)
  3. Y. Ohta, Y. Tsukada and T. Sugimori, J. Biochem., 106, 1086 (1989)
  4. L. Warren, J. Biol. Chem., 234, 1971 (1959)

Ordering information

Product Name Grade Storage Product No. PKG Size
Neuraminidase from Arthrobacter ureafaciens, highly purified SP R 24229-61 1 unit
24229-74 5 units

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Others

Sialic Acid derivative

Ordering information

Product Name CAS No. Formula M.W. Purity Storage Product No. PKG Size
N-Acetylneuraminic Acid, methyl ester(Sialic acid, methyl ester) 22900-11-4 C12H21NO9 323.30 95%(TLC) F inquiry 2G
inquiry 10G
N-Acetylneuraminic Acid, 2,4,7,8,9-pentaacetate
(Sialic acid, 2,4,7,8,9-pentaacetate)		 4887-11-0 C21H29NO14 519.45 95%(TLC) F inquiry 2G
inquiry 10G
N-Acetylneuraminic Acid, methyl ester, 2,4,7,8,9-pentaacetate
(Sialic acid, methyl ester, 2,4,7,8,9-pentaacetate)		 73208-82-9 C22H31NO14 533.48 95%(TLC) F inquiry 1G
inquiry 10G
N-Acetyl-2-phenylthioneuraminic acid, methyl ester,
4,7,8,9-tetraacetate
(2-(SPh)-sialic acid, methyl ester, 4,7,8,9-tetraacetate)		 155155-64-9 C26H33NO12S 583.61 (α, β mix)
98%(TLC)		 RT inquiry 500MG
inquiry 1G
inquiry 10G
N-Iodosuccinimide 516-12-1 C4H4INO2 224.98 99%(TLC) R inquiry 1G
inquiry 5G
2-O-Methyl α-D-N-acetylneuraminic acid 50930-22-8 C12H21NO9 323.30 98%(TLC) F inquiry 10MG
inquiry 50MG
inquiry 100MG
2-O-Phenyl α-D-N-acetylneuraminic acid 15964-32-6 C17H23NO9 385.37 98%(TLC) F inquiry 10MG
inquiry 50MG
inquiry 100MG
Methyl 2,3-didehydro-4,7,8,9-tetera-O-acetyl-N-acetylneuraminate 73960-72-2 C20H27NO12 473.42 - R inquiry 100MG

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Glucose derivative

Glucose derivative

Ordering information

Product Name CAS No. Formula M.W. Purity Storage Product No. PKG Size
4-Methoxyphenyl 2,3,4,6-tetra-O-acetyl-β-Dglucopyranoside 14581-81-8 C21H26O11 454.42 98%(TLC) R inquiry 10G
4-Methoxyphenyl β-D-glucopyranoside 6032-32-2 C13H18O7 286.28) 98%(TLC) R inquiry 5G
Phenyl 2,3,4,6-tetra-O-acetyl-1-thio-β-D-glucopyranoside 23661-28-1 C20H24O9S 440.46 98%(TLC) R inquiry 5G
Phenyl 1-thio-β-D-glucopyranoside 2936-70-1 C12H16O5S 272.32 98%(TLC) R inquiry 1G
5G
Ethyl 2,3,4,6-tetra-O-acetyl-1-thio-
β-D-glucopyranoside		 52645-73-5 C16H24O9S 392.42 98%(TLC) R inquiry 1G
5G
Ethyl 1-thio-β-D-glucopyranoside 7473-36-1 C8H16O5S 224.28 98%(TLC) R inquiry 1G
5G

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Galactose derivative

Galactose derivative

Ordering information

Product Name CAS No. Formula M.W. Purity Storage Product No. PKG Size
4-Methoxyphenyl 2,3,4,6-tetra-O-acetyl-β-Dglucopyranoside 14581-81-8 C21H26O11 454.42 98%(TLC) R inquiry 10G
4-Methoxyphenyl β-D-glucopyranoside 6032-32-2 C13H18O7 286.28) 98%(TLC) R inquiry 5G
Phenyl 2,3,4,6-tetra-O-acetyl-1-thio-β-D-glucopyranoside 23661-28-1 C20H24O9S 440.46 98%(TLC) R inquiry 5G
Phenyl 1-thio-β-D-glucopyranoside 2936-70-1 C12H16O5S 272.32 98%(TLC) R inquiry 1G
5G
Ethyl 2,3,4,6-tetra-O-acetyl-1-thio-
β-D-glucopyranoside		 52645-73-5 C16H24O9S 392.42 98%(TLC) R inquiry 1G
5G
Ethyl 1-thio-β-D-glucopyranoside 7473-36-1 C8H16O5S 224.28 98%(TLC) R inquiry 1G
5G

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Lactose derivative

Lactose derivative

Ordering information

Product Name CAS No. Formula M.W. Purity Storage Product No. PKG Size
4-Methoxyphenyl 2,3,4,6-tetra-O-acety-β-D-galactopyranoside 2872-65-3 C21H26O11 454.42 98%(TLC) R inquiry 10G
4-Methoxyphenyl β-D-galactopyranoside 3150-20-7 C13H18O7 286.28 98%(TLC) R inquiry 5G
4-Methoxyphenyl 2,6-di-O-benzyl-β-D-galactopyranoside 159922-50-6 C27H30O7 466.52 98%(TLC) R inquiry 2G
4-Methoxyphenyl 2,3-di-O-benzyl-β-D-galactopyranoside - C27H30O7 466.52 98%(TLC) R inquiry 1G
Phenyl 2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside 24404-53-3 C20H24O9S 440.47 98%(TLC) R inquiry 5G
Phenyl 1-thio-β-D-galactopyranoside 16758-34-2 C12H16O5S 272.32 98%(TLC) R inquiry 1G
inquiry 5G

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Protected sialo-containing oligosaccharides

Protected sialo-containing oligosaccharides

Ordering information

Product Name CAS No. Formula M.W. Purity Storage Product No. PKG Size
Protected 6'-α-Sialylgalactose;
Protected NeuAc a(2→6)-D-Gal		 - C47H57O19 939.95 98%(TLC) R inquiry 10MG
inquiry 100MG
inquiry 1G
Protected 3'-α-Sialylgalactose;
Protected NeuAc a(2→3)-D-Gal		 - C47H57O19 939.95 98%(TLC) R inquiry 10MG
inquiry 100MG
inquiry 1G
Protected 6'-β-Sialylgalactose;
Protected NeuAc β(2→6)-D-Gal		 - C47H57O19 939.95 98%(TLC) R inquiry 10MG
inquiry 100MG

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Hyaluronic acid

Hyaluronic acid

Ordering information

Product Name Storage Product No. PKG Size
Hyaluronic Acid from Streptococcus R inquiry 5G
inquiry 25G
Hyaluronic Acid Sodium Salt from Streptococcus R inquiry 5G
inquiry 25G
Hyaluronic Acid Sodium Salt from Rooster Comb R inquiry 2G
inquiry 5G
inquiry 25G

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze