Products

TMB Solution for Western Blotting

TMB (tetramethyl benzidine) solution is used with horseradish peroxidase (HRP)-based western blotting procedure, but not applicable for ELISA or immunohistochemistry.


Features

Product Images

  • Convenience: No CDD-camera-equipped imager or x-ray film needed
  • Sensitivity: Low Picogram level
  • Safety: Does not contain DAB or o-Dianisidine
  • Ready to use: Does not need to dilute

Application 1

Detection for Gly m4, a major soybean allergen

CBB Staining Western blotting
cbb.png western.png

Experimental details

Sample Soy milk, dilution ratio as above
Detection TMB Solution for Western Blotting; 5 minutes reaction time

Data courtesy of Professor Tatsuya Moriyama and Dr. Erika Yano, Faculty of Agriculture, Department of Applied Biological Chemistry, Kinki University

Reference data 1

Comparison with other picogram-level chemiluminescence kits and colorimetric substrates

TMB Solution for Western Blotting; incubation time,10 minutes
TMB solution for WB.jpg TMB Solution for Western Blotting
Other colorimetric substrates / incubation time,10 minutes
Peroxidase-Stain-Kit.png Peroxidase Stain Kit(#26652-70)
Peroxidase-Stain-DAB-Kit.png Peroxidase Stain DAB Kit(#25985-50)
2297b083b4038238fe98c306e48e9f94.png Peroxidase Stain DAB Kit + Metal Enhancer for DAB Stain(#07388-24)
Chemiluminescence kits / exposure time,10 minutes
Chemi-Lumi-One-L.png Chemi-Lumi One L(#07880)
ECL.png Product A from competitor
ECL-Start.png Product B from competitor

Experimental details

Sample Hela cell extraction, 2 times dilution started from 12 ug
Primary antibody Anti-β-actin mAb manufactured by MBL, #177-3, X 5,000 dilution
Secondary antibody Anti-mouse IgG, HRP-linked whole Ab Sheep manufactured by GE, #NA931, X 10,000 dilution
Detector for chemiluminescence LAS-3000 Super mode

Reference data 2

Quantitative analysis

seinoukakunin_en.JPG

Experimental details

Sample GST-His protein, 1.5 times dilution series started from 600 ng
Primary antibody Anti-GST (mouse IgGa-k) Monoclonal(GS019) manufactured by Nacalai, #04435, X 2,000 dilution
Secondary antibody Anti-mouse IgG, HRP-linked whole Ab Sheep manufactured by GE, #NA931, X 10,000 dilution
Detection TMB Solution for Western Blotting; 5 minutes incubation time
Analysis Determination of band density with ImageJ software by Seiko Epson

Application 2

Mid-femtogram-level sensitivity achieved with TMB Solution for Western Blotting along with Signal Enhancer HIKARI

TMB Solution for Western Blotting; incubation time, 10 minutes
TMB-for-WB-HIKARI.png TMB Solution for Western Blotting + Signal Enhancer HIKARI for Western Blotting and ELISA(#02270-81)
Chemiluminescence kits / exposure time, 10 minutes
Chemi-Lumi-One-Super.png Chemi-Lumi One Super(#02230)
Clarity(B).png Product C from competitor

Experimental details

Sample Hela cell extraction, 2 times dilution started from 3 ug
Primary antibody Anti-GAPDH manufactured by Novous, #NB300-322, X 5,000 dilution
Secondary antibody Anti-rabbit IgG, HRP-linked whole Ab Donkey manufactured by GE, #NA934, X 10,000 dilution
Detector for chemiluminescence LAS-3000 Super mode

Video

TMB Solution for Western Blotting (5 minute incubation)

Download

TMB Solution for Western Blottingpdf(PDF 1.25 MB)

Ordering information

Product Name Grade Storage Catalog Number PKG Size Price
TMB Solution for Western Blotting SP 4°C 18186-24 200 ml e-Nacalai