We are pleased to announce that a paper summarizing a method for the separation and high-sensitivity detection of all structural isomers and enantiomers of amino acids using a novel optical labeling reagent L-FDVDA and a COSMOSIL 3C18-EB column has been accepted for publication in the "Analytical Methods" journal by the Royal Society of Chemistry. It is now available for early access.

Paper title:

Simultaneous separation and identification of all structural isomers and enantiomers of aminobutyric acid using a highly sensitive chiral resolution labeling reagent

Authors:

Makoto Ozaki¹, Motoshi Shimotsuma¹, Takefumi Kuranaga², Hideaki Kakeya², Tsunehisa Hirose¹

Affiliations:

1: Separation and Purification Group, Research Dept., Nacalai Tesque, Inc.
2: Department of System Chemotherapy and Molecular Sciences, Division of Medicinal Frontier Sciences, Graduate School of Pharmaceutical Sciences, Kyoto University

Journal:

Analytical Methods, 2023, Accepted Manuscript
DOI: https://doi.org/10.1039/D3AY01665J

Summary:

Aminobutyric acid has five structural isomers and three enantiomers, each with its unique functionality. Therefore, there is a need for methods to separate and identify these. However, a simple method for separating and identifying all structural isomers and enantiomers and detecting them with high sensitivity using LC-MS has not been established until now. In this paper, we have established a method for separation and identification using LC-MS; after derivatization (labeling) with our optical separation labeling reagent L-FDVDA, HPLC analysis is done using a conventional C₁₈ column. Furthermore, we were able to detect aminobutyric acid in human, mouse, and goat serum with higher sensitivity than existing chiral labeling agents, demonstrating differences in the aminobutyric acid contained in different animal species.

Product information:

>> Optical labeling reagent L-FDVDA