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Separation of amyloid β fragment peptides using DL-Amino Acid Labeling Kit

We are pleased to announce the below study using our DL-amino acid labeling kit, made in collaboration with Professor Hideaki Kakeya from Graduate School of Pharmaceutical Sciences, Kyoto University, was selected for inclusion in HOT Articles 2023 by the Royal Society of Chemistry journal Analyst.

Paper: Separation of amyloid β fragment peptides with racemised and isomerised aspartic acid residues using an original chiral resolution labeling reagent
Author: Makoto Ozaki, Motoshi Shimotsuma1, Takefumi Kuranaga2, Hideaki Kakeya2, Tsunehisa Hirose1
Affiliation: 1Research and Development Department, Purification Section, Nacalai Tesque, Inc.,
2Department of System Chemotherapy and Molecular Sciences, Division of Medicinal Frontier Sciences,
Graduate School of Pharmaceutical Sciences, Kyoto University
Article: Analyst, 2023, Advance Article
Overview: Amyloid β (Aβ, a total length of 42 residues) is a causal substance ofAlzheimer's disease (AD), caused by the accumulation of senile plaques in the brain and resulting in cognition function decline. It is reported that Aβ shows cytotoxicity and is highly cohesive bythe racemisation and isomerisation of residues 1, 7, and 23 of aspartic acid(Asp) in Aβ. This study shows the development of a method to separate and identify racemised and isomerised Asp residues in Aβ by using a labeling reagent D-FDLDA and liquid chromatography-mass spectrometry (LC-MS) with an ordinary C18 column. We believe that the method in this study will contribute to the early diagnosis of or the elucidation of AD and could be used to identify racemisation and isomerisation of disease DL-amino acid_Amyloid.png
Previous study: M. Ozaki, T. Kuwayama, T. Hirose, M. Shimotsuma, A. Hashimoto, T. Kuranaga, H. Kakeya
Separation and identification of the DL-forms of short-chain peptides using a new chiral resolution labeling reagent
Anal. Bioanal.Chem., 414, 4039-4046 (2022).
Product information: DL-Amino Acid Labeling Kit