Saccharide Separation Columns
Saccharides are not retained on standard C18 columns because of their low hydrophobicity. COSMOSIL Sugar-D and NH2-MS are specifi cally designed for separation of saccharides. COSMOSIL C18-PAQ is recommended for hydrophobic glycosides or saccharide derivatives.
|Silica Gel||High Purity Porous Spherical Silica|
|Average Particle Size||5 µm|
|Average Pore Size||-||approx. 120Å|
|Specific Surface Area||-||approx. 300 m2/g|
|Bonded Phase Stracture||-|
|Stationary Phase||Secondary/tertiary amine||Aminopropyl group|
|Target Substances||Monosaccharides, oligosaccharides|
|End Capping Treatment||Near-perfect Treatment|
|Carbon Content||-||approx. 4%|
Comparison of Retention
The conventional aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5%-10% as shown below.
|Column Size||4.6 mm I.D. x 250 mm||Detection||RI|
|Flow Rate||1.0 ml/min||Sample||