Saccharide Separation Columns

Saccharides are not retained on standard C18 columns because of their low hydrophobicity. COSMOSIL Sugar-D and NH2-MS are specifi cally designed for separation of saccharides. COSMOSIL C18-PAQ is recommended for hydrophobic glycosides or saccharide derivatives.


Packing Material Sugar-D NH2-MS
USP Code   L8 
Silica Gel High Purity Porous Spherical Silica
Average Particle Size 5 µm
Average Pore Size - approx. 120Å
Specific Surface Area - approx. 300 m2/g
Bonded Phase Stracture -
Stationary Phase  Secondary/tertiary amine  Aminopropyl group
Bonding Type  Polymeric
Target Substances Monosaccharides, oligosaccharides
End Capping Treatment Near-perfect Treatment 
Carbon Content - approx. 4%
  • First choice for saccharide analysis
  • High durability
  • Good quantitative analysis
  • Different selectivity from Sugar-D

Comparison of Retention

The conventional aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5%-10% as shown below.

Column Size 4.6 mm I.D. x 250 mm Detection RI
Flow Rate 1.0 ml/min Sample
  1. D-(+)-Glucose
  2. Maltose
Temperature 30°C