Products

bFGF Solution, Human, Recombinant (154 a.a.), Animal-origin free

bFGF is a recombinant protein expressed by Corynebacterium glutamicum. This gram-positive bacteria does not produce toxins such as endotoxin. Therefore, it has been used for amino acid production for food and pharmaceutical industry for more than 50 years.

*Manufactured by AJINOMOTO CO., INC



Features

  • High quality bFGF designed for cell culture
  • Endotoxin tested (below 1 EU/μg) / sterilized by filtration
  • Ready-to-use liquid form
  • Animal-origin free

Purity

HPLC analysis

bFGF_HPLC application.PNG

Separation condition
Column COSMOSIL Protein-R
4.6 mmI.D.-150 mm (#06526-21)
Temperature 30℃
Mobile phase

A:0.05%TFA-20%Acetonitrile
B:0.05%TFA-50% Acetonitrile
B conc. 0→100% 20 min Linear gradient

Detector

UV 220 nm, 0.128 AUFS

Flow rate 1.0 mL/min Injection volume 1.5 µg

Cosmosil is a trademark of Nacalai Tesque, Inc.

By analysis of bFGF monomer by HPLC, our product has the highest monomer purity of those.

Specification

  • Sequence:
    1
    AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG
    51
    VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC
    101
    FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM
    151
    SAKS
  • Format: PBS solution with 1 mmol/L DTT
    *bFGF concentration differs depending on production batch. Ask us if you need this information.
  • Activity: Tested with stem cell culture
    *not disclosed test method
  • Purity: 95% or greater by SDS-PAGE

Download

bFGF Solution, Human, Recombinant (154 a.a.), Animal-origin freepdf(PDF 1.16 MB)

Ordering Information

Product Name Storage Catalog Number PKG Size Price
bFGF(basic FGF) Solution, Human, Recombinant(154 a.a.), Animal-Free -80°C 19155-07 10 μg e-Nacalai
19155-36 5x10 μg
19155-81 10 mg

Related product

Product Name Storage Catalog Number PKG Size Price
Activin A Solution, Human, Recombinant -80°C 18585-36 10 μg e-Nacalai
18585-94 50 μg
18585-81 1 mg