RNA Switch™ series
RNA SwitchTM series is product series utilizing "RNA Switch technology" which was invented by Prof. Hirohide Saito who works at CiRA, Kyoto University. RNA Switch can regulate expression of genes in a cell by detecting microRNA (miRNA) which exists in a cell and have various roles for cellular mechanisms. One of the major features is that cells can distinguish the active state of miRNAs in living cells and it can be used for regenerative medicine by using an RNA switch for cell-type specific active miRNAs identified by this tool. It enables identification and selection of differentiated target cells, such as cardiomyocytes, and application as a searching tool for disease-related miRNAs.
RNA SwitchTM is an artificially synthesized mRNA that contains an array that recognizes miRNAs and a marker gene. This series enables identification and selection of cells by using marker genes such as fluorescent proteins and suicide genes. It is possible to purify only specific cells without using a cell sorter or other devices and can be easily introduced into cells by the same process as normal transfection.
Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches.
Cell Stem Cell. 2015 May 20. pii: S1934-5909(15)00163-0. doi: 10.1016/j.stem.2015.04.005. PMID: 26004781
Miki K, Endo K, Takahashi S, Funakoshi S, Takei I, Katayama S, Toyoda T, Kotaka M, Takaki T, Umeda M, Okubo C, Nishikawa M, Oishi A, Narita M, Miyashita I, Asano K, Hayashi K, Osafune K, Yamanaka S, Saito H, Yoshida Y.
MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells.
Scientific Reports. 2016 Sep 9;6:32532. doi: 10.1038/srep32532. PMID: 27608814 Parr CJ, Katayama S, Miki K, Kuang Y, Yoshida Y, Morizane A, Takahashi J, Yamanaka S, Saito H.
|Control detector RNA SwitchTM||P-0001||9μg||-80℃|
※The amount guarantees minimum 9μg.
RNA SwitchTM series is registered trademark of aceRNA Technologies Co., Ltd.